Colorimetric Chymotrypsin Substrate I

Product Description

Colorimetric Chymotrypsin Substrate I
Catalog number: B2013117
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 mg
Molecular Weight or Concentration: 499.5 g/mol
Supplied as: Lyophilized Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: Suc-GGF-pNA, Suc-Gly-Gly-Phe-pNA, Suc-Gly-Gly-Phe-p-Nitroanilide
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: McCartney CE, MacLeod JA, Greer PA, Davies PL. An easy-to-use FRET protein substrate to detect calpain cleavage in vitro and in vivo Biochim Biophys Acta Mol Cell Res. 2018 Feb;1865(2):221-230.
2: Boehnke N, Maynard HD. Design of modular dual enzyme-responsive peptides Biopolymers. 2017 Sep;108(5):10.1002/bip.23035.
3: Rinderknecht H, Fleming RM. A new, highly sensitive and specific assay for chymotrypsin Clin Chim Acta. 1975 Mar 10;59(2):139-46.
4: Shibao PY, Anjolette FA, Lopes NP, Arantes EC. First serine protease inhibitor isolated from Rhinella schneideri poison J Venom Anim Toxins Incl Trop Dis. 2015 Aug 13;21:30.
5: Yakoby N, Raskin I. A simple method to determine trypsin and chymotrypsin inhibitory activity J Biochem Biophys Methods. 2004 Jun 30;59(3):241-51.
6: Gupta R, Chen J, Srivastava OP. A serine-type protease activity of human lens βA3-crystallin is responsible for its autodegradation Mol Vis. 2010 Nov 2;16:2242-52.
7: Evensen NA, Braun PC. The effects of tea polyphenols on Candida albicans: inhibition of biofilm formation and proteasome inactivation Can J Microbiol. 2009 Sep;55(9):1033-9.
8: Huang C, Wei P, Fan K, Liu Y, Lai L. 3C-like proteinase from SARS coronavirus catalyzes substrate hydrolysis by a general base mechanism Biochemistry. 2004 Apr 20;43(15):4568-74.
9: Niinobe M, Hitomi Y, Fujii S. A sensitive colorimetric assay for various proteases using naphthyl ester derivatives as substrates J Biochem. 1980 Mar;87(3):779-83.
10: Lopes AR, Sato PM, Terra WR. Insect chymotrypsins: chloromethyl ketone inactivation and substrate specificity relative to possible coevolutional adaptation of insects and plants Arch Insect Biochem Physiol. 2009 Mar;70(3):188-203.

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