Brilliant Blue G (Highly Pure)

Product Description

Brilliant Blue G (Highly Pure)
Catalog number: B2010643
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 g
Molecular Weight or Concentration: 854.02 g/mol
Supplied as: Powder
Applications: SDS-PAGE, protein quantification and more
Storage: RT
Keywords: Coomassie Brilliant Blue G, Acid blue 90
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Georgiou CD, Grintzalis K, Zervoudakis G, Papapostolou I. Mechanism of
Coomassie brilliant blue G-250 binding to proteins: a hydrophobic assay for
nanogram quantities of proteins. Anal Bioanal Chem. 2008 May;391(1):391-403.

2: Kielkopf CL, Bauer W, Urbatsch IL. Bradford Assay for Determining Protein
Concentration. Cold Spring Harb Protoc. 2020 Apr 1;2020(4):102269.

3: Azuma K, Noda Y, Hirasawa K, Ueta T. BRILLIANT BLUE G-ASSISTED INTERNAL
LIMITING MEMBRANE PEELING FOR MACULAR HOLE: A Systematic Review of Literature
and Meta-Analysis. Retina. 2016 May;36(5):851-8.

4: Ernst O, Zor T. Linearization of the bradford protein assay. J Vis Exp. 2010
Apr 12;(38):1918.

5: Pereira JMS, Barreira AL, Gomes CR, Ornellas FM, Ornellas DS, Miranda LC,
Cardoso LR, Coutinho-Silva R, Schanaider A, Morales MM, Leite M Jr, Takiya CM.
Brilliant blue_G, a P2X7 receptor antagonist, attenuates early phase of renal
inflammation, interstitial fibrosis and is associated with renal cell
proliferation in ureteral obstruction in rats. BMC Nephrol. 2020 May
29;21(1):206.

6: Kruger NJ. The Bradford method for protein quantitation. Methods Mol Biol.
1994;32:9-15.

7: Sakamoto K, Inukai M, Mori A, Nakahara T. Brilliant Blue_G protects against
photoreceptor injury in a murine endotoxin-induced uveitis model. Exp Eye Res.
2018 Dec;177:45-49.

8: Totan Y, Güler E, Dervişoğulları MS. Brilliant Blue G assisted epiretinal
membrane surgery. Sci Rep. 2014 Feb 10;4:3956.

9: Estrada JA, Ducrocq GP, Kim JS, Kaufman MP. Intrathecal injection of
brilliant blue G, a P2X7 antagonist, attenuates the exercise pressor reflex in
rats. Am J Physiol Regul Integr Comp Physiol. 2020 Aug 1;319(2):R223-R232.
10: Simonian MH. Spectrophotometric determination of protein concentration. Curr
Protoc Cell Biol. 2002 Aug;Appendix 3:Appendix 3B.

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