ALX1 Double Nickase Plasmid

Product Description

ALX1 Double Nickase Plasmid
Catalog number: B2015424
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 µg
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: 2-8 °C
Keywords: ALX1 Double Nickase Plasmid (h)
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
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7: Renouf B, Piganeau M, Ghezraoui H, Jasin M, Brunet E. Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks Methods Enzymol. 2014;546:251-71.
8: Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, Karlin-Neumann GA, Conklin BR. Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing Sci Rep. 2016 Mar 31;6:23549.
9: Horzempa J, Shanks RM, Brown MJ, Russo BC, O’Dee DM, Nau GJ. Utilization of an unstable plasmid and the I-SceI endonuclease to generate routine markerless deletion mutants in Francisella tularensis J Microbiol Methods. 2010 Jan;80(1):106-8.
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