WST-1 (Highly Pure)


Catalog Number: B2012343 (10 mg)
WST-1 (Highly Pure) is a high quality water-soluble and cell-permeable tetrazolium dye. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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SKU: B2012343 Categories: , Tag:


WST-1 (Highly Pure)
Catalog number: B2012343
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 mg
Molecular Weight or Concentration: 651.3 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: 5-(2,4-disulfophenyl)-2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Scarcello E, Lambremont A, Vanbever R, Jacques PJ, Lison D. Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese PLoS One. 2020 Apr 16;15(4):e0231634.
2: Lutter AH, Scholka J, Richter H, Anderer U. Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures Clin Hemorheol Microcirc. 2017;67(3-4):327-342.
3: Calibasi-Kocal G, Pakdemirli A, Bayrak S, Ozupek NM, Sever T, Basbinar Y, Ellidokuz H, Yigitbasi T. Curcumin effects on cell proliferation, angiogenesis and metastasis in colorectal cancer J BUON. 2019 Jul-Aug;24(4):1482-1487.
4: Joo KM, Kim S, Koo YJ, Lee M, Lee SH, Choi D, Lim KM. Development and validation of UPLC method for WST-1 cell viability assay and its application to MCTT HCE™ eye irritation test for colorful substances Toxicol In Vitro. 2019 Oct;60:412-419.
5: Hu HF, Xu WW, Li YJ, He Y, Zhang WX, Liao L, Zhang QH, Han L, Yin XF, Zhao XX, Pan YL, Li B, He QY. Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission Theranostics. 2021 Jan 1;11(4):1828-1844.
6: Datar R, Perrin G, Chalansonnet V, Perry A, Perry JD, van Belkum A, Orenga S. Automated antimicrobial susceptibility testing of slow-growing Pseudomonas aeruginosa strains in the presence of tetrazolium salt WST-1 J Microbiol Methods. 2021 Jul;186:106252.
7: Ngamwongsatit P, Banada PP, Panbangred W, Bhunia AK. WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line J Microbiol Methods. 2008 Jun;73(3):211-5.
8: Yin LM, Wei Y, Wang WQ, Wang Y, Xu YD, Yang YQ. Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells Biol Res. 2014 Dec 22;47(1):75.
9: Shin SP, Sohn HC, Jin CN, Lee J. Evaluation of the effect of protease inhibitors on the viability of Miamiensis avidus using the WST-1 assay Parasitol Int. 2020 Jun;76:102093.
10: Peskin AV, Winterbourn CC. Assay of superoxide dismutase activity in a plate assay using WST-1 Free Radic Biol Med. 2017 Feb;103:188-191.

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