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UltraFast Biotinylation Kit (ug scale, 10 reactions)

$750.00 $585.00

Catalog Number: K2010011L (microgram scale, 10 reactions)
UltraFast Antibody and Protein Biotinylation Kit (microgram scale, 10 reactions) is a high quality research product used as for custom protein and antibody biotinylation in vitro. 45 min ultrafast and highly efficient protocol. This kit is for the biotinylation of microgram amounts (10-1000 ug). The kit contains all the required materials for 10 independent biotinylation reactions. Components include NHS-activated Biotin Linkers, biotinylation and storage buffers as well as  size-exlcusion columns for the removal of free unconjugated biotin. Kit has been validated in applications that include ELISA, CLIA, enzymatic tagging and more. Custom bulk orders of this product are available upon request.

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SKU: K2010011L Category:

Description

UltraFast Antibody and Protein Biotinylation Kit (microgram scale, 10 reactions)
Catalog number: K2010011L
Lot number: Batch Dependent
Expiration Date: Batch dependent
Size: 10 reactions
Supplied as: Ready-to-use kit with all required conjugation material

Storage: 2-8°C

Applications: for custom protein and antibody biotinylation in vitro. 45 min ultrafastand highly efficient protocol. This kit is for the biotinylation of microgram amounts (10-1000 ug). The kit contains all the required materials for 10 independent biotinylation reactions. Components include NHS-activated Biotin Linkers, biotinylation and storage buffers as well as  size-exlcusion columns for the removal of free unconjugated biotin. Kit has been validated in applications that include ELISA, CLIA, enzymatic tagging and more.

Keywords: Biotinylation Kits, Biotin labelling kit, biotin tagging kit, biotin conjugation kit
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: De Munter S, Görnemann J, Derua R, Lesage B, Qian J, Heroes E, Waelkens E,
Van Eynde A, Beullens M, Bollen M. Split-BioID: a proximity biotinylation assay
for dimerization-dependent protein interactions. FEBS Lett. 2017
Jan;591(2):415-424.

2: Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA, Ting AY.
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in
living human cells by proximity biotinylation. Elife. 2017 Apr 25;6:e24463.

3: Kaewsapsak P, Shechner DM, Mallard W, Rinn JL, Ting AY. Live-cell mapping of
organelle-associated RNAs via proximity biotinylation combined with protein-RNA
crosslinking. Elife. 2017 Dec 14;6:e29224.

4: Beckett D, Kovaleva E, Schatz PJ. A minimal peptide substrate in biotin
holoenzyme synthetase-catalyzed biotinylation. Protein Sci. 1999 Apr;8(4):921-9.

5: Rice GM, Nutiu R, Gampe CM. Direct Chemical Biotinylation of RNA 5′-Ends
Using a Diazo Reagent. Methods Mol Biol. 2019;1870:81-87.

6: Fouda AE, Embogama DM, Ramanayake-Mudiyanselage V, H Pflum MK. Chitosan-
assisted permeabilization of ATP-biotin for live cell kinase-catalyzed
biotinylation. Biotechniques. 2018 Sep;65(3):143-148.

7: Trinh LA, Chong-Morrison V, Sauka-Spengler T. Biotagging, an in vivo
biotinylation approach for cell-type specific subcellular profiling in
zebrafish. Methods. 2018 Nov 1;150:24-31.

8: Varnaitė R, MacNeill SA. Meet the neighbors: Mapping local protein
interactomes by proximity-dependent labeling with BioID. Proteomics. 2016
Oct;16(19):2503-2518.

Products Related to UltraFast Antibody and Protein Biotinylation Kit (microgram scale, 10 reactions): Research Kits

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