Transcription Buffer 5X (Molecular Biology Grade)
$295.00
Catalog Number: B2010102 (1 mL)
Transcription Buffer 5X is an optimized, reproducible buffer for the In vitro transcription reactions with SP6, T7 or T3 RNA polymerase. Custom bulk orders are available upon request.
In stock
Description
Transcription Buffer 5X (Molecular Biology Grade)
| Catalog number: | B2010102 |
| Lot number: | Batch Dependent |
| Expiration date: | Batch Dependent |
| Volume/Weight | 1 mL |
| pH | 7.9 |
| Supplied as | 5X concentrate |
| Appearance | Clear solution |
| Applications | In vitro transcription reactions with SP6, T7 or T3 RNA polymerase. |
| Shelf-Life | 1 year from date of manufacture |
| Storage: | Keep at 2-8°C |
| Keywords: | SP6 RNA Polymerase Buffer, T7 RNA Polymerase Buffer, T3 RNA Polymerase Buffer, RNA Polymerase Buffer |
| Grade | Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. |
| References | 1: Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986 May 5;189(1):113-30. 2: Borkotoky S, Murali A. The highly efficient T7 RNA polymerase: A wonder 3: Gholamalipour Y, Karunanayake Mudiyanselage A, Martin CT. 3′ end additions by 4: Sarcar SN, Miller DL. A specific, promoter-independent activity of T7 RNA 5: Liang X, Li C, Wang W, Li Q. Integrating T7 RNA Polymerase and Its Cognate 6: Milisavljevič N, Perlíková P, Pohl R, Hocek M. Enzymatic synthesis of base- 7: Jin H, Lindblad P, Bhaya D. Building an Inducible T7 RNA Polymerase/T7 8: Ji S, Thomforde J, Rogers C, Fu I, Broyde S, Tretyakova NY. Transcriptional 9: Juhas M, Ajioka JW. T7 RNA polymerase-driven inducible cell lysis for DNA 10: Hassan MI, McSorley FR, Hotta K, Boddy CN. Inducible T7 RNA Polymerase- |
| Products Related to Transcription Buffer 5X | Biochemical Buffers and Solutions |
Additional information
| Weight | 48 oz |
|---|---|
| Dimensions | 8 × 8 × 8 in |
Ordering Information
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