Transcription Buffer 5X (Molecular Biology Grade)


Catalog Number: B2010102 (1 mL)
Transcription Buffer 5X is an optimized, reproducible buffer for the In vitro transcription reactions with SP6, T7 or T3 RNA polymerase. Custom bulk orders are available upon request.

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Transcription Buffer 5X (Molecular Biology Grade)

Catalog number: B2010102
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 1 mL
pH 7.9
Supplied as 5X concentrate
Appearance Clear solution
Applications In vitro transcription reactions with SP6, T7 or T3 RNA polymerase.
Shelf-Life 1 year from date of manufacture
Storage: Keep at 2-8°C
Keywords: SP6 RNA Polymerase Buffer, T7 RNA Polymerase Buffer, T3 RNA Polymerase Buffer, RNA Polymerase Buffer
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
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3: Gholamalipour Y, Karunanayake Mudiyanselage A, Martin CT. 3′ end additions by
T7 RNA polymerase are RNA self-templated, distributive and diverse in character-
RNA-Seq analyses. Nucleic Acids Res. 2018 Oct 12;46(18):9253-9263.

4: Sarcar SN, Miller DL. A specific, promoter-independent activity of T7 RNA
polymerase suggests a general model for DNA/RNA editing in single subunit RNA
Polymerases. Sci Rep. 2018 Sep 17;8(1):13885.

5: Liang X, Li C, Wang W, Li Q. Integrating T7 RNA Polymerase and Its Cognate
Transcriptional Units for a Host-Independent and Stable Expression System in
Single Plasmid. ACS Synth Biol. 2018 May 18;7(5):1424-1435.

6: Milisavljevič N, Perlíková P, Pohl R, Hocek M. Enzymatic synthesis of base-
modified RNA by T7 RNA polymerase. A systematic study and comparison of
5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside
triphosphates as substrates. Org Biomol Chem. 2018 Aug 15;16(32):5800-5807.

7: Jin H, Lindblad P, Bhaya D. Building an Inducible T7 RNA Polymerase/T7
Promoter Circuit in Synechocystis sp. PCC6803. ACS Synth Biol. 2019 Apr

8: Ji S, Thomforde J, Rogers C, Fu I, Broyde S, Tretyakova NY. Transcriptional
Bypass of DNA-Protein and DNA-Peptide Conjugates by T7 RNA Polymerase. ACS Chem
Biol. 2019 Dec 20;14(12):2564-2575.

9: Juhas M, Ajioka JW. T7 RNA polymerase-driven inducible cell lysis for DNA
transfer from Escherichia coli to Bacillus subtilis. Microb Biotechnol. 2017

10: Hassan MI, McSorley FR, Hotta K, Boddy CN. Inducible T7 RNA Polymerase-
mediated Multigene Expression System, pMGX. J Vis Exp. 2017 Jun 27;(124):55187.
protein synthesis reveals insights into the regulation of T7 RNA p

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