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RNA Sample Loading Buffer (Molecular Biology Grade)

$150.00

Catalog Number: B2010118 (4 x 1 mL)
RNA Sample Loading Buffer is a high quality research product used as RNA loading buffer during RNA electrophoresis. Custom bulk orders of this product are available upon request.

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SKU: B2010118 Categories: ,

Description

RNA Sample Loading Buffer
Catalog number: B2010118
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 4 x 1 mL
pH: n/a
Supplied as: Concentrate (Dilute 1:3 or 1:6 with sample before use)
Appearance: Blue Solution
Applications: RNA loading buffer during RNA electrophoresis.
Storage: −20°C
Keywords: RNA loading buffer, RNA electrophoresis buffer
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct
selective high-level expression of cloned genes. J Mol Biol. 1986 May
5;189(1):113-30.

2: Borkotoky S, Murali A. The highly efficient T7 RNA polymerase: A wonder
macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118(Pt
A):49-56.

3: Gholamalipour Y, Karunanayake Mudiyanselage A, Martin CT. 3′ end additions by
T7 RNA polymerase are RNA self-templated, distributive and diverse in character-
RNA-Seq analyses. Nucleic Acids Res. 2018 Oct 12;46(18):9253-9263.

4: Sarcar SN, Miller DL. A specific, promoter-independent activity of T7 RNA
polymerase suggests a general model for DNA/RNA editing in single subunit RNA
Polymerases. Sci Rep. 2018 Sep 17;8(1):13885.

5: Liang X, Li C, Wang W, Li Q. Integrating T7 RNA Polymerase and Its Cognate
Transcriptional Units for a Host-Independent and Stable Expression System in
Single Plasmid. ACS Synth Biol. 2018 May 18;7(5):1424-1435.

6: Milisavljevič N, Perlíková P, Pohl R, Hocek M. Enzymatic synthesis of base-
modified RNA by T7 RNA polymerase. A systematic study and comparison of
5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside
triphosphates as substrates. Org Biomol Chem. 2018 Aug 15;16(32):5800-5807.

7: Jin H, Lindblad P, Bhaya D. Building an Inducible T7 RNA Polymerase/T7
Promoter Circuit in Synechocystis sp. PCC6803. ACS Synth Biol. 2019 Apr
19;8(4):655-660.

8: Ji S, Thomforde J, Rogers C, Fu I, Broyde S, Tretyakova NY. Transcriptional
Bypass of DNA-Protein and DNA-Peptide Conjugates by T7 RNA Polymerase. ACS Chem
Biol. 2019 Dec 20;14(12):2564-2575.

9: Juhas M, Ajioka JW. T7 RNA polymerase-driven inducible cell lysis for DNA
transfer from Escherichia coli to Bacillus subtilis. Microb Biotechnol. 2017
Nov;10(6):1797-1808.

10: Hassan MI, McSorley FR, Hotta K, Boddy CN. Inducible T7 RNA Polymerase-
mediated Multigene Expression System, pMGX. J Vis Exp. 2017 Jun 27;(124):55187.
protein synthesis reveals insights into the regulation of T7 RNA p

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