RIPA Lysis and Extraction Buffer

$95.00

Catalog Number: B2010077 (100 mL)
RIPA Lysis and Extraction Buffer is an efficient and rapid lysis of cells (including mammalian cells). The buffer is useful for extracting proteins from the cytoplasm and the nucleus as well as various cellular membranes. This buffer is compatible with most protein purification and assaying protocols. Custom bulk orders are available upon request.

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SKU: B2010077 Categories: ,

Description

RIPA Lysis and Extraction Buffer

Catalog number: B2010077
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 100 mL
pH 7.6
Supplied as Ready-to-use 1X solution
Appearance Clear solution
Applications Efficient and rapid lysis of various types of cells (including mammalian cells). The buffer is useful for extracting proteins from the cytoplasme and the nucleus as well as various cellular membranes. This buffer is compatible with most protein purification and assaying protocols.
Shelf-Life 2 years from date of manufacture
Storage: Keep at 2-8°C
Keywords: RIPA Buffer, RIPA Lysis Buffer, RIPA Extraction Buffer
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References Related to RIPA Lysis Buffer 1: de Fries RU, Golub SH. Characteristics and mechanism of IFN-gamma-induced
protection of human tumor cells from lysis by lymphokine-activated killer cells.
J Immunol. 1988 May 15;140(10):3686-93.2: Thiele DL, Lipsky PE. Tumor cell lysis by T cells distinct from NK cells and
alloantigen-specific cytotoxic T cells. Clin Immunol Immunopathol. 1988
Dec;49(3):405-23.3: Collins JL, Kao MS. The anticancer drug, cisplatin, increases the naturally
occurring cell-mediated lysis of tumor cells. Cancer Immunol Immunother.
1989;29(1):17-22.4: Manning LS, Bowman RV, Darby SB, Robinson BW. Lysis of human malignant
mesothelioma cells by natural killer (NK) and lymphokine-activated killer (LAK)
cells. Am Rev Respir Dis. 1989 Jun;139(6):1369-74.

5: Bean P, Mazumder A. Resistance of different tumor cells to lysis by
lymphokine activated killer cells can be mediated by distinct mechanisms.
Immunobiology. 1992 Jun;185(1):63-77.

6: Kondo S, Miyatake S, Kikuchi H, Oda Y, Iwasaki K, Ohyama K, Namba Y.
Mechanism of interferon gamma-induced protection of human gliosarcoma cells from
lymphokine-activated killer lysis: division of lymphokine-activated killer cells
into natural killer- and T-like cells. Neurosurgery. 1992 Sep;31(3):534-40.

7: Borysiewicz LK, Rodgers B, Morris S, Graham S, Sissons JG. Lysis of human
cytomegalovirus infected fibroblasts by natural killer cells: demonstration of
an interferon-independent component requiring expression of early viral proteins
and characterization of effector cells. J Immunol. 1985 Apr;134(4):2695-701.

8: Fresa KL, Karjalainen HE, Tevethia SS. Sensitivity of simian virus
40-transformed C57BL/6 mouse embryo fibroblasts to lysis by murine natural
killer cells. J Immunol. 1987 Feb 15;138(4):1215-20.

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