RBC Lysis Buffer 10X (Biotechnology Grade)

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RBC Lysis Buffer 10X
T7 IgG Conjugated to DyLight 650

RBC Lysis Buffer 10X (Biotechnology Grade)

$195.00

Catalog Number: B2010090 (50 mL)
RBC Lysis Buffer 10X is optimized for the lysis of Red Blood Cells (RBC, erythrocytes) as found in the spleen or human peripheral blood. Ammonium chloride-based, it has minimal effect on lymphocytes and nucleated RBC. Dilute to 1X with di-water before use. Custom bulk orders are available upon request.

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SKU: B2010090 Categories: ,

Description

RBC Lysis Buffer 10X

Catalog number: B2010090
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 50 mL
pH N/A
Supplied as 10X
Appearance Clear solution
Applications This buffer is optimized for the lysis of Red Blood Cells (RBC, erythrocytes) as found in the spleen or human peripheral blood. Ammonium chloride-based, it has minimal effect on lymphocytes and nucleated RBC.
Shelf-Life 1 year from date of manufacture
Storage: Keep at 2-8°C
Keywords: RBC lysis, erythrocytes lysis buffer, red blood cells lysis buffer, rbc lysis buffer protocol
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References 1: Buoro S, Vavassori M, Pipitone S, Benegiamo A, Lochis E, Fumagalli S, Falanga
A, Marchetti M, Crippa A, Ottomano C, Lippi G. Evaluation of nucleated red blood
cell count by Sysmex XE-2100 in patients with thalassaemia or sickle cell
anaemia and in neonates. Blood Transfus. 2015 Oct;13(4):588-94.2: Rosse WF, Dourmashkin R, Humphrey JH. Immune lysis of normal human and
paroxysmal nocturnal hemoglobinuria (PNH) red blood cells. 3. The membrane
defects caused by complement lysis. J Exp Med. 1966 Jun 1;123(6):969-84.3: Mauritz JM, Esposito A, Ginsburg H, Kaminski CF, Tiffert T, Lew VL. The
homeostasis of Plasmodium falciparum-infected red blood cells. PLoS Comput Biol.
2009 Apr;5(4):e1000339.4: Grisendi G, Finetti E, Manganaro D, Cordova N, Montagnani G, Spano C, Prapa
M, Guarneri V, Otsuru S, Horwitz EM, Mari G, Dominici M. Detection of
microparticles from human red blood cells by multiparametric flow cytometry.
Blood Transfus. 2015 Apr;13(2):274-80.

5: van der Spuy WJ, Pretorius E. Interaction of red blood cells adjacent to and
within a thrombus in experimental cerebral ischaemia. Thromb Res.
2013;132(6):718-23.
6: Dalibalta S, Ellory JC, Browning JA, Wilkins RJ, Rees DC, Gibson JS. Novel
permeability characteristics of red blood cells from sickle cell patients
heterozygous for HbS and HbC (HbSC genotype). Blood Cells Mol Dis. 2010 Jun
15;45(1):46-52.
7: Górnicki A. The hemolysis kinetics of psoriatic red blood cells. Blood Cells
Mol Dis. 2008 Sep-Oct;41(2):154-7.
8: Chow S, Hedley D, Grom P, Magari R, Jacobberger JW, Shankey TV. Whole blood
fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations.
Cytometry A. 2005 Sep;67(1):4-17.
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