Pyruvate Oxidase (purified from microorganisms)

Product Description

Pyruvate Oxidase (purified from microorganisms)
Catalog number: B2010235
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 1 mg
pH: na
Supplied as: Ready-to-use
Appearance: Lyophilized Powder
Applications: high purity (95%), high specific activity (10U/mg) dried powder of Pyruvate Oxidase, an enzyme useful for enzymatic determination of pyruvate, GOT and GPT levels in clinical samples.
Storage: -20C
Keywords: oxygen oxidoreductase
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Lu J, Zhao Y, Zhang J. High-level expression of Aerococcus viridans pyruvate
oxidase in Escherichia coli by optimization of vectors and induction conditions.
Lett Appl Microbiol. 2018 Sep;67(3):262-269.

2: Bryant JC, Dabbs RC, Oswalt KL, Brown LR, Rosch JW, Seo KS, Donaldson JR,
McDaniel LS, Thornton JA. Pyruvate oxidase of Streptococcus pneumoniae
contributes to pneumolysin release. BMC Microbiol. 2016 Nov 9;16(1):271.

3: Cornacchione LP, Klein BA, Duncan MJ, Hu LT. Interspecies Inhibition of
Porphyromonas gingivalis by Yogurt-Derived Lactobacillus
delbrueckii Requires Active Pyruvate Oxidase. Appl Environ Microbiol. 2019
Aug 29;85(18):e01271-19.

4: Echlin H, Frank MW, Iverson A, Chang TC, Johnson MD, Rock CO, Rosch JW.
Pyruvate Oxidase as a Critical Link between Metabolism and Capsule Biosynthesis
in Streptococcus pneumoniae. PLoS Pathog. 2016 Oct 19;12(10):e1005951.

5: Liang J, Zhao J, Wang Z, Wang Y. Temperature gradient-based high-cell density
fed-batch fermentation for the production of pyruvate oxidase by recombinant E.
coli. Prep Biochem Biotechnol. 2018 Feb 7;48(2):188-193.

6: Brissac T, Shenoy AT, Patterson LA, Orihuela CJ. Cell Invasion and Pyruvate
Oxidase-Derived H₂O₂ Are Critical for Streptococcus
pneumoniae-Mediated Cardiomyocyte Killing. Infect Immun. 2017 Dec
19;86(1):e00569-17.

7: Taniai H, Iida K, Seki M, Saito M, Shiota S, Nakayama H, Yoshida S. Concerted
action of lactate oxidase and pyruvate oxidase in aerobic growth of
Streptococcus pneumoniae: role of lactate as an energy source. J Bacteriol. 2008
May;190(10):3572-9. doi: 10.1128/JB.01882-07. Epub 2008 Mar 14. PMID: 18344365;
PMCID: PMC2395018.

8: Carvalho SM, Farshchi Andisi V, Gradstedt H, Neef J, Kuipers OP, Neves AR,
Bijlsma JJ. Pyruvate oxidase influences the sugar utilization pattern and
capsule production in Streptococcus pneumoniae. PLoS One. 2013 Jul
3;8(7):e68277.

9: Goffin P, Muscariello L, Lorquet F, Stukkens A, Prozzi D, Sacco M,
Kleerebezem M, Hols P. Involvement of pyruvate_oxidase activity and acetate
production in the survival of Lactobacillus plantarum during the stationary
phase of aerobic growth. Appl Environ Microbiol. 2006 Dec;72(12):7933-40.

10: Belanger AE, Clague MJ, Glass JI, Leblanc DJ. Pyruvate_oxidase is a
determinant of Avery’s rough morphology. J Bacteriol. 2004 Dec;186(24):8164-71.

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