Protein G Agarose (lyophilized powder)

Description

Protein G Agarose (lyophilized)
Catalog number: B2010489
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 mg
Molecular Weight or Concentration: na
Supplied as: Lyophilized powder
Applications: a tool in affinity chromatography, protein chromatography and antibody purification
Storage: −20°C
Keywords: Protein G–Agarose
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
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protein A and protein G Sepharose. Methods Mol Biol. 2010;588:33-41.

2: Akerström B, Brodin T, Reis K, Björck L. Protein G: a powerful tool for
binding and detection of monoclonal and polyclonal antibodies. J Immunol. 1985
Oct;135(4):2589-92.

3: Hirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM,
Haugland RP. Easily reversible desthiobiotin binding to streptavidin, avidin,
and other biotin-binding proteins: uses for protein labeling, detection, and
isolation. Anal Biochem. 2002 Sep 15;308(2):343-57.

4: Zarrineh M, Mashhadi IS, Farhadpour M, Ghassempour A. Mechanism of antibodies
purification by protein A. Anal Biochem. 2020 Nov 15;609:113909.

5: Hehemann JH, Correc G, Barbeyron T, Helbert W, Czjzek M, Michel G. Transfer
of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota.
Nature. 2010 Apr 8;464(7290):908-12.

6: Bonifacino JS, Dell’Angelica EC, Springer TA. Immunoprecipitation. Curr
Protoc Immunol. 2001 May;Chapter 8:Unit 8.3.

7: Salehi N, Peng CA. Purification of CD47-streptavidin fusion protein from
bacterial lysate using biotin-agarose affinity chromatography. Biotechnol Prog.
2016 Jul 8;32(4):949-58.

8: Wang Y, Zhang X, Han N, Wu Y, Wei D. Oriented covalent immobilization of
recombinant protein A on the glutaraldehyde activated agarose support. Int J
Biol Macromol. 2018 Dec;120(Pt A):100-108.

9: Bonifacino JS, Dell’Angelica EC, Springer TA. Immunoprecipitation. Curr
Protoc Mol Biol. 2001 May;Chapter 10:Unit 10.16.
10: Kawabata SI, Shibata T. Purification and Assays of Tachycitin. Methods Mol
Biol. 2020;2132:317-323.

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