N-Hydroxysuccinimide NHS

Product Description

N-Hydroxysuccinimide NHS
Catalog number: B2010676
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 g
Molecular Weight or Concentration: 115.09 g/mol
Supplied as: Powder
Applications: synthesis of N-hydroxysuccinimide ester via a dehydration reaction in the presence of dicyclohexylcarbodiimide
Storage: RT
Keywords: NHS, 1-Hydroxy-2,5-pyrrolidinedione, HOSu
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Berg EA, Fishman JB. Labeling Antibodies with N-Hydroxy_succinimide-Long Chain (NHS-LC)-Biotin Cold Spring Harb Protoc. 2020 Jan 2;2020(1):099259.
2: Berg EA, Fishman JB. Labeling Antibodies Using N-Hydroxy_succinimide (NHS)-Fluorescein Cold Spring Harb Protoc. 2019 Mar 1;2019(3).
3: Jiang H, D’Agostino GD, Cole PA, Dempsey DR. Selective protein N-terminal labeling with N-hydroxysuccinimide esters Methods Enzymol. 2020;639:333-353.
4: Jiang H, Cole PA. N-Terminal Protein Labeling with N-Hydroxy_succinimide Esters and Microscale Thermophoresis Measurements of Protein-Protein Interactions Using Labeled Protein Curr Protoc. 2021 Jan;1(1):e14.
5: Menzel C, Hauser M, Frey A, Jelkmann M, Laffleur F, Götzfried SK, Gust R, Bernkop-Schnürch A. Covalently binding mucoadhesive polymers: N-hydroxysuccinimide grafted polyacrylates Eur J Pharm Biopharm. 2019 Jun;139:161-167.
6: Fischer MJ. Amine coupling through EDC/NHS: a practical approach Methods Mol Biol. 2010;627:55-73.
7: Spataru T , Radu MM , Spataru N , Fujishima A . Voltammetric determination of N-hydroxysuccinimide at conductive diamond electrodes Analyst. 2018 May 15;143(10):2356-2362.
8: Ishikawa S, Matsukuma D, Iijima K, Iijima M, Osawa S, Otsuka H. N-Hydroxysuccinimide Bifunctionalized Triblock Cross-Linker Having Hydrolysis Property for a Biodegradable and Injectable Hydrogel System ACS Biomater Sci Eng. 2019 Nov 11;5(11):5759-5769.
9: Dempsey DR, Jiang H, Kalin JH, Chen Z, Cole PA. Site-Specific Protein Labeling with N-Hydroxysuccinimide-Esters and the Analysis of Ubiquitin Ligase Mechanisms J Am Chem Soc. 2018 Aug 1;140(30):9374-9378.
10: Gao Y, Li SG, Liu Q, Liu SS, Ye L, Song ZJ, Du WD. Establishment of a 1, 4, 7, 10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono-N-hydroxysuccinimide ester (DOTA-NHS-ester) based lectin microarray for efficiently detecting serum glycans in gastric cancers Anal Biochem. 2020 May 15;597:113686.

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