Mouse DHFR Protein

Description

Mouse DHFR Protein
Catalog number: B2012120
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 µg
Molecular Weight or Concentration: 1 mg/mL
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: -20 °C
Keywords: EC 1.5.1.3, Dihydrofolate reductase protein
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
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2: Mensah EO, Guo XY, Gao XD, Fujita M. Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins J Biosci Bioeng. 2019 Oct;128(4):487-494.
3: Singh P, Abeysinghe T, Kohen A. Linking protein motion to enzyme catalysis Molecules. 2015 Jan 13;20(1):1192-209.
4: Suzuki S, Hatano Y, Yoshii T, Tsukiji S. Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPT Methods Mol Biol. 2021;2312:237-251.
5: Linn AK, Samainukul N, Sakdee S, Angsuthanasombat C, Katzenmeier G. A Helicobacter pylori Vacuolating Cytotoxin A: Mouse DHFR Fusion Protein Triggers Dye Release from Liposomes Curr Microbiol. 2018 Feb;75(2):223-230.
6: Oh JH, Chen SJ, Varshavsky A. A reference-based protein degradation assay without global translation inhibitors J Biol Chem. 2017 Dec 29;292(52):21457-21465.
7: Westwood AD, Rowe DA, Clarke HR. Improved recombinant protein yield using a codon deoptimized DHFR selectable marker in a CHEF1 expression plasmid Biotechnol Prog. 2010 Nov-Dec;26(6):1558-66.
8: Kampmeyer C, Larsen-Ledet S, Wagnkilde MR, Michelsen M, Iversen HKM, Nielsen SV, Lindemose S, Caregnato A, Ravid T, Stein A, Teilum K, Lindorff-Larsen K, Hartmann-Petersen R. Disease-linked mutations cause exposure of a protein quality control degron Structure. 2022 Sep 1;30(9):1245-1253.e5.
9: Shen CC, Sung LY, Lin SY, Lin MW, Hu YC. Enhancing Protein Production Yield from Chinese Hamster Ovary Cells by CRISPR Interference ACS Synth Biol. 2017 Aug 18;6(8):1509-1519.
10: Takano K, Arai S, Sakamoto S, Ushijima H, Ikegami T, Saikusa K, Konuma T, Hamachi I, Akashi S. Screening of protein-ligand interactions under crude conditions by native mass spectrometry Anal Bioanal Chem. 2020 Jul;412(17):4037-4043.

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