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Laemmli Lysis Buffer (Biotechnology Grade)

Original price was: $595.00.Current price is: $295.00.

Catalog Number: B2010127 (4 x 1 mL)
Laemmli Lysis buffer is a high quality research product used as denaturing and loading protein samples during SDS-PAGE (wihtout reducing agents). Custom bulk orders of this product are available upon request.

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Product Description

Laemmli Lysis buffer
Catalog number: B2010127
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 4 x 1 mL
pH: 6.8
Supplied as: Ready-to-use solution
Appearance: Blue Solution
Applications: denaturing and loading protein samples during SDS-PAGE (wihtout reducing agents).
Storage: −20°C
Keywords: Laemmli Buffer, SDS-PAGE loading buffer, Protein Denaturing buffer
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Karlsson JO, Ostwald K, Kåbjörn C, Andersson M. A method for protein assay in
Laemmli buffer. Anal Biochem. 1994 May 15;219(1):144-6.2: Sheen H. Quantitation of yeast total proteins in sodium dodecyl sulfate-
polyacrylamide gel electrophoresis sample buffer for uniform loading. Anal
Biochem. 2016 Apr 1;498:95-7.3: Amponsah A, Nayak B. Evaluation of the efficiency of three extraction
conditions for the immunochemical detection of allergenic soy proteins in
different food matrices. J Sci Food Agric. 2018 Apr;98(6):2378-2384.4: Cai X, Zheng Y, Speck NA. A Western Blotting Protocol for Small Numbers of
Hematopoietic Stem Cells. J Vis Exp. 2018 Aug 22;(138):56855.

5: Scully RR, Pressley TA, O’Neil RG. A site-directed antibody recognizes a
component of the ouabain-binding domain of the alpha 1 subunit of rat
Na+,K(+)-ATPase. Biochem Cell Biol. 1993 Nov-Dec;71(11-12):538-43.

6: Guthrie CR, Murray LW, Kopchok GE, Rosenbaum D, White RA. Biochemical
mechanisms of laser vascular tissue fusion. J Invest Surg. 1991;4(1):3-12.

7: Rendić D, Wilson IB, Lubec G, Gutternigg M, Altmann F, Léonard R. Adaptation
of the “in-gel release method” to N-glycome analysis of low-milligram amounts of
material. Electrophoresis. 2007 Dec;28(23):4484-92.

8: Wang X, Wang H, Wang D, Wang D, Han B, Tian W, Guo A. Thermal denaturation
produced degenerative proteins and interfered with MS for proteins dissolved in
lysis buffer in proteomic analysis. Electrophoresis. 2011 Feb;32(3-4):348-56.

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Additional Information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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