IPTG Solution 100 mM (Biotechnology Grade)

Description

100 mM IPTG Solution
Catalog number: B2010137
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 10 x 1 mL
pH: 7
Supplied as: Ready-to-use solution
Appearance: Clear solution
Applications: optimized stock solution of IPTG (isopropyl-beta-D-thiogalactopyranoside), a synthetic analog of lactose. IPTG inactivates the lac repressor of the lac operon, trigeering the induction of beta-galactosidase synthesis. IPTG is used with X-Gal in the blue/white colony screening. it also used to induce the expression of recombinant protein under the control of the lac operon.
Storage: “”-20°C””
Keywords: IPTG solution, IPTG inducer, IPTG expression, IPTG X-Gal
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Tran DTM, Phan TTP, Huynh TK, Dang NTK, Huynh PTK, Nguyen TM, Truong TTT,
Tran TL, Schumann W, Nguyen HD. Development of inducer-free expression plasmids
based on IPTG-inducible promoters for Bacillus subtilis. Microb Cell Fact. 2017
Jul 25;16(1):130.
2: Marbach A, Bettenbrock K. lac operon induction in Escherichia coli:
Systematic comparison of IPTG and TMG induction and influence of the
transacetylase LacA. J Biotechnol. 2012 Jan;157(1):82-8.

3: Wandrey G, Bier C, Binder D, Hoffmann K, Jaeger KE, Pietruszka J, Drepper T,
Büchs J. Light-induced gene expression with photocaged IPTG for induction
profiling in a high-throughput screening system. Microb Cell Fact. 2016 Apr
23;15:63.
4: Kjaerulff S, Nielsen O. An IPTG-inducible derivative of the fission yeast nmt
promoter. Yeast. 2015 Jun;32(6):469-78.

5: Dvorak P, Chrast L, Nikel PI, Fedr R, Soucek K, Sedlackova M, Chaloupkova R,
de Lorenzo V, Prokop Z, Damborsky J. Exacerbation of substrate toxicity by IPTG
in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway. Microb
Cell Fact. 2015 Dec 21;14:201.
6: Zhang Z, Kuipers G, Niemiec Ł, Baumgarten T, Slotboom DJ, de Gier JW, Hjelm
A. High-level production of membrane proteins in E. coli BL21(DE3) by omitting
the inducer IPTG. Microb Cell Fact. 2015 Sep 16;14:142.

7: Goodson KA, Wang Z, Haeusler AR, Kahn JD, English DS. LacI-DNA-IPTG loops:
equilibria among conformations by single-molecule FRET. J Phys Chem B. 2013 Apr
25;117(16):4713-22.
8: Ravishankar S, Ambady A, Ramu H, Mudugal NV, Tunduguru R, Anbarasu A, Sharma
UK, Sambandamurthy VK, Ramaiah S. An IPTG Inducible Conditional Expression
System for Mycobacteria. PLoS One. 2015 Aug 6;10(8):e0134562.

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