Imidazole (Biotechnology Grade)


Catalog Number: B2010035 (5 g)
Imidazole (≥99.0% pure). Multi-purpose compound with several biotechnoly applications. Useful for buffer formulation during his-tagged protein purification, immobilized metal-affinity chromatography (IMAC). Custom bulk orders are available upon request.

In stock

SKU: B2010035 Category: Tag:


Imidazole (Biotechnology Grade)

Catalog number: B2010035
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 5 g
Molecular Formula C3H4N2
Molecular Weight 68.08
CAS Number 288-32-4
Application: his-tagged protein purification, immobilized metal-affinity chromatography (IMAC)
Shelf-Life 2 years from date of manufacture
Storage: Room temperature
Keywords: 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References 1: Burton RF. Temperature and acid-base balance in ectothermic vertebrates: the
imidazole alphastat hypotheses and beyond. J Exp Biol. 2002 Dec;205(Pt

2: Eseola AO, Obi-Egbedi NO. Spectroscopic study of 2-, 4- and 5-substituents on
pKa values of imidazole heterocycles prone to intramolecular proton-electrons
transfer. Spectrochim Acta A Mol Biomol Spectrosc. 2010 Feb;75(2):693-701.
3: Bhaumik J, Yao Z, Borbas KE, Taniguchi M, Lindsey JS. Masked imidazolyl-
dipyrromethanes in the synthesis of imidazole-substituted porphyrins. J Org
Chem. 2006 Nov 10;71(23):8807-17.

4: Souza GR, Levin CS, Hajitou A, Pasqualini R, Arap W, Miller JH. In vivo
detection of gold-imidazole self-assembly complexes: NIR-SERS signal reporters.
Anal Chem. 2006 Sep 1;78(17):6232-7. doi: 10.1021/ac060483a. PMID: 16944906.

5: Ames WM, Larsen SC. DFT calculations of the EPR parameters for Cu(ii) DETA
imidazole complexes. Phys Chem Chem Phys. 2009 Oct 1;11(37):8266-74.

6: Marboutin L, Desbois A, Berthomieu C. Low-frequency heme, iron-ligand, and
ligand modes of imidazole and imidazolate complexes of iron protoporphyrin and
microperoxidase in aqueous solution. An analysis by far-infrared difference
spectroscopy. J Phys Chem B. 2009 Apr 2;113(13):4492-9.

7: Robertson DW, Krushinski JH, Beedle EE, Leander JD, Wong DT, Rathbun RC.
Structure-activity relationships of (arylalkyl)imidazole anticonvulsants:
comparison of the (fluorenylalkyl)imidazoles with nafimidone and denzimol. J Med
Chem. 1986 Sep;29(9):1577-86.

8: Katsumoto S, Smith SM, Martasek P, Salerno JC. Competition and binding of
arginine, imidazole, and aminoguanidine to endothelial nitric oxide synthase:
aminoguanidine is a poor model for substrate, intermediate, and arginine analog
inhibitor binding. Nitric Oxide. 2003 May;8(3):149-54.

9: Schilling S, Niestroj AJ, Rahfeld JU, Hoffmann T, Wermann M, Zunkel K,
Wasternack C, Demuth HU. Identification of human glutaminyl cyclase as a
metalloenzyme. Potent inhibition by imidazole derivatives and heterocyclic
chelators. J Biol Chem. 2003 Dec 12;278(50):49773-9.

Related Products Biochemical Buffers and Solutions

Ordering Information

1- Order online using our website’s automated shopping cart and checkout system (available 24/7).

2- Order by email: (we accept electronic PO, available 24/7).

3- Order by Fax: 1-858-437-9884 (available 24/7).

4- Order by phone: 1-858-900-3210 (6 am – 8 pm PST).

5- Order by Text/SMS: 1-858-900-3210 (6 am – 8 pm PST).