HEPES (≥99.5%, Biotechnology Grade)


Catalog Number: B2010032 (50 g)
HEPES (≥99.5% pure). Multi-purpose buffer for most biotechnology applications. Buffering range between pH 6.8 to 8.2. Useful for buffer formulation, reagent formulation, tissue culture and more. Custom bulk orders are available upon request.

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SKU: B2010032 Category: Tag:


HEPES (≥99.5% pure powder)

Catalog number: B2010032
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 50 g
Molecular Formula C8H18N2O4S
Molecular Weight 238.3
CAS Number 7365-45-9
Application: buffer formulation, reagent formulation, reagent stabilization
Shelf-Life 2 years from date of manufacture
Storage: Room temperature
Keywords: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References 1: Morgia F, Torti M, Montigiani M, Piscitelli C, Giallonardo A, Schimberni M,
Giannini P, Sbracia M. Use of a medium buffered with N-hydroxyethylpiperazine-N-
ethanesulfonate (HEPES) in intracytoplasmic sperm injection procedures is
detrimental to the outcome of in vitro fertilization. Fertil Steril. 2006
2: Simpson JA, Cheeseman KH, Smith SE, Dean RT. Free-radical generation by
copper ions and hydrogen peroxide. Stimulation by Hepes buffer. Biochem J. 1988
Sep 1;254(2):519-23.3: Kasraian K, Kuzniar A, Earley D, Kamicker BJ, Wilson G, Manion T, Hong J,
Reiber C, Canning P. Sustained in vivo activity of recombinant bovine
granulocyte colony stimulating factor (rbG-CSF) using HEPES buffer. Pharm Dev
Technol. 2001 Aug;6(3):441-7.

4: Habib A, Tabata M. Oxidative DNA damage induced by HEPES
(2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) buffer in the presence
of Au(III). J Inorg Biochem. 2004 Nov;98(11):1696-702.

5: Vasconcelos MT, Azenha MA, Lage OM. Electrochemical evidence of surfactant
activity of the hepes pH buffer which may have implications on trace metal
availability to cultures in vitro. Anal Biochem. 1996 Oct 15;241(2):248-53.

6: Lomonosova EE, Kirsch M, Rauen U, de Groot H. The critical role of Hepes in
SIN-1 cytotoxicity, peroxynitrite versus hydrogen peroxide. Free Radic Biol Med.
1998 Mar 1;24(4):522-8.

7: Kirsch M, Lomonosova EE, Korth HG, Sustmann R, de Groot H. Hydrogen peroxide
formation by reaction of peroxynitrite with HEPES and related tertiary amines.
Implications for a general mechanism. J Biol Chem. 1998 May 22;273(21):12716-24.

8: Cowan AI, Martin RL. Simultaneous measurement of pH and membrane potential in
rat dorsal vagal motoneurons during normoxia and hypoxia: a comparison in
bicarbonate and HEPES buffers. J Neurophysiol. 1995 Dec;74(6):2713-21.

9: Svajger U, Jeras M. Optimal dendritic cell differentiation in rpmi media
requires the absence of HEPES buffer. Immunol Invest. 2011;40(4):413-26.

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Additional information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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