ELISA Blocking Buffer (10X Solution)

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ELISA Blocking Buffer
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ELISA Blocking Buffer (10X Solution)

Original price was: $795.00.Current price is: $395.00.

Catalog Number: B2010010 (100 mL)
ELISA Blocking Buffer (10X Solution) is a concentrated reagent designed to saturate unoccupied binding sites on ELISA plate surfaces, effectively reducing non-specific binding and background noise. This 100 mL solution improves assay sensitivity and accuracy by ensuring that primary and secondary antibodies bind only to their specific targets. It can also serve as a stabilizer for coated biomolecules and as a diluent for antibodies to ensure optimal reagent concentrations during the immunoassay process.

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Product Description

4.9/5 - (14 votes)

ELISA Blocking Buffer (10X)

Catalog number: B2010010
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume: 100 mL
Appearance: Clear to slightly white Solution
Application: ELISA
Shelf-Life 3 years
Storage: Room temperature
Keywords: Elisa blocking protocol, ELISA blocking buffer, blocking Elisa plates, blocking buffer Elisa.

ELISA Coating/Washing/Blocking Protocol

Detailed protocol is provided.

References

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  • 2: Enzyme-linkedimmunosorbentassay (ELISA). Reen DJ. Methods Mol Biol. 1994;32:461-6.
  • 3: Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection. Arya SK, Estrela P. Sensors (Basel). 2018 Jun 22;18(7):2010.
  • 4: The application of immunoassay techniques, including enzyme-linkedimmunosorbentassay (ELISA), to snake venom research. Theakston RD. Toxicon. 1983;21(3):341-52.
  • 5: Commercial enzyme-linked immunosorbent assay versuspolymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis. Brasil PE, Castro R, Castro Ld. Mem Inst Oswaldo Cruz. 2016 Jan;111(1):1-19.
  • 6: Lequin RM. Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay
    (ELISA). Clin Chem. 2005 Dec;51(12):2415-8.
  • 7: Aydin S. A short history, principles, and types of ELISA, and our laboratory
    experience with peptide/protein analyses using ELISA. Peptides. 2015
    Oct;72:4-15.
  • 8: Eble JA. Titration ELISA as a Method to Determine the Dissociation Constant
    of Receptor Ligand Interaction. J Vis Exp. 2018 Feb 15;(132):57334.
  • 9: Pang B, Zhao C, Li L, Song X, Xu K, Wang J, Liu Y, Fu K, Bao H, Song D, Meng
    X, Qu X, Zhang Z, Li J. Development of a low-cost paper-based ELISA method for
    rapid Escherichia coli O157:H7 detection. Anal Biochem. 2018 Feb 1;542:58-62.
  • 10: Major M, Law M. Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA.
    Methods Mol Biol. 2019;1911:421-432. doi: 10.1007/978-1-4939-8976-8_28.

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Additional Information

Weight 5.5 oz
Dimensions 5 × 3 × 3 in
4.9/5 - (14 votes)

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