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Deoxynucleoside Triphosphate Set for PCR

$595.00

Catalog Number: B2012453 (4 X 250 μL)
Deoxynucleoside Triphosphate Set for PCR is a high quality a set of four 100 mM solutions of dATP, dGTP, dCTP and dTTP oprimized for PCR applications. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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Description

Deoxynucleoside Triphosphate Set for PCR
Catalog number: B2012453
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 4 X 250 μL
Molecular Weight or Concentration: 100 mM
Supplied as: Liquid Set
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: datp, dctp, dgtp, dttp, set of datp, dctp, dgtp, and dttp, pcr grade
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7.
2: Fox RM. Changes in deoxynucleoside triphosphate pools induced by inhibitors and modulators of ribonucleotide reductase Pharmacol Ther. 1985;30(1):31-42.
3: Setterholm NA, Haratipour P, Kashemirov BA, McKenna CE, Joyce GF. Kinetic Effects of β,γ-Modified Deoxynucleoside 5′-Triphosphate Analogues on RNA-Catalyzed Polymerization of DNA Biochemistry. 2021 Jan 12;60(1):1-5.
4: Landoni JC, Wang L, Suomalainen A. Quantitative solid-phase assay to measure deoxynucleoside triphosphate pools Biol Methods Protoc. 2018 Oct 11;3(1):bpy011.
5: Tomori T, Nagaoka K, Takeshita L, Shiozawa T, Miyatake Y, Masaki Y, Sekine M, Seio K. Deoxynucleoside Triphosphate Containing Pyridazin-3-one Aglycon as a Thymidine Triphosphate Substitute for Primer Extension and Chain Elongation by Klenow Fragments J Org Chem. 2018 Aug 3;83(15):8353-8363.
6: Wang HY, Hsin P, Huang CY, Chang ZF. A Convenient and Sensitive Method for Deoxynucleoside Triphosphate Quantification by the Combination of Rolling Circle Amplification and Quantitative Polymerase Chain Reaction Anal Chem. 2021 Oct 26;93(42):14247-14255.
7: Ye Y, Muller JG, Burrows CJ. Synthesis and characterization of the oxidized dGTP lesions spiroiminodihydantoin-2′-deoxynucleoside-5′- triphosphate and guanidinohydantoin-2′-deoxynucleoside-5′- triphosphate J Org Chem. 2006 Mar 3;71(5):2181-4.
8: Ding Y, Ou L, Ding Q. Enzymatic Synthesis of Nucleoside Triphosphates and Deoxynucleoside Triphosphates by Surface-Displayed Kinases Appl Biochem Biotechnol. 2020 Apr;190(4):1271-1288.
9: Goldstone DC, Ennis-Adeniran V, Hedden JJ, Groom HC, Rice GI, Christodoulou E, Walker PA, Kelly G, Haire LF, Yap MW, de Carvalho LP, Stoye JP, Crow YJ, Taylor IA, Webb M. HIV-1 restriction factor SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase Nature. 2011 Nov 6;480(7377):379-82.
10: Huang CY, Yagüe-Capilla M, González-Pacanowska D, Chang ZF. Quantitation of deoxynucleoside triphosphates by click reactions Sci Rep. 2020 Jan 17;10(1):611.

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