Cas9 Protein

Product Description

Cas9 Protein
Catalog number: B2016726
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 µg
Molecular Weight or Concentration: 161.2 kDa
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -80°C
Keywords: Recombinant Cas9 Protein
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Carmignotto GP, Azzoni AR. On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains J Biotechnol. 2019 Dec 20;306:62-70.
2: Singpant P, Tubsuwan A, Sakdee S, Ketterman AJ, Jearawiriyapaisarn N, Kurita R, Nakamura Y, Songdej D, Tangprasittipap A, Bhukhai K, Chiangjong W, Hongeng S, Saisawang C. Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells Protein Expr Purif. 2023 Oct;210:106313.
3: Yu X, Liang X, Xie H, Kumar S, Ravinder N, Potter J, de Mollerat du Jeu X, Chesnut JD. Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX Biotechnol Lett. 2016 Jun;38(6):919-29.
4: Xue C, Greene EC. DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing Trends Genet. 2021 Jul;37(7):639-656.
5: Liang X, Potter J, Kumar S, Zou Y, Quintanilla R, Sridharan M, Carte J, Chen W, Roark N, Ranganathan S, Ravinder N, Chesnut JD. Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection J Biotechnol. 2015 Aug 20;208:44-53.
6: Albadri S, Del Bene F, Revenu C. Genome editing using CRISPR/Cas9-based knock-in approaches in zebrafish Methods. 2017 May 15;121-122:77-85.
7: Seki A, Rutz S. Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells J Exp Med. 2018 Mar 5;215(3):985-997.
8: Truong DJ, Kühner K, Kühn R, Werfel S, Engelhardt S, Wurst W, Ortiz O. Development of an intein-mediated split-Cas9 system for gene therapy Nucleic Acids Res. 2015 Jul 27;43(13):6450-8.
9: Laughery MF, Wyrick JJ. Simple CRISPR-Cas9 Genome Editing in Saccharomyces cerevisiae Curr Protoc Mol Biol. 2019 Dec;129(1):e110.
10: Haapaniemi E, Botla S, Persson J, Schmierer B, Taipale J. CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response Nat Med. 2018 Jul;24(7):927-930.

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