Bradford Reagent

Product Description

Bradford Reagent
Catalog number: B2012932
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 250 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: 2-8°C
Keywords: Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Kielkopf CL, Bauer W, Urbatsch IL. Bradford Assay for Determining Protein Concentration Cold Spring Harb Protoc. 2020 Apr 1;2020(4):102269.
2: Kruger NJ. The Bradford method for protein quantitation Methods Mol Biol. 1994;32:9-15.
3: Ernst O, Zor T. Linearization of the bradford protein assay J Vis Exp. 2010 Apr 12;(38):1918.
4: Noble JE, Bailey MJ. Quantitation of protein Methods Enzymol. 2009;463:73-95.
5: Goldring JPD. Measuring Protein Concentration with Absorbance, Lowry, Bradford Coomassie Blue, or the Smith Bicinchoninic Acid Assay Before Electrophoresis Methods Mol Biol. 2019;1855:31-39.
6: Cossarizza A, Chang HD, Radbruch A, Acs A, Adam D, Adam-Klages S, Agace WW, Aghaeepour N, Akdis M, Allez M, Almeida LN, Alvisi G, Anderson G, Andrä I, Annunziato F, Anselmo A, Bacher P, Baldari CT, Bari S, Barnaba V, Barros-Martins J, Battistini L, Bauer W, Baumgart S, Baumgarth N, Baumjohann D, Baying B, Bebawy M, Becher B, Beisker W, Benes V, Beyaert R, Blanco A, Boardman DA, Bogdan C, Borger JG, Borsellino G, Boulais PE, Bradford JA, Brenner D, Brinkman RR, Brooks AES, Busch DH, Büscher M, Bushnell TP, Calzetti F, Cameron G, Cammarata I, Cao X, Cardell SL, Casola S, Cassatella MA, Cavani A, Celada A, Chatenoud L, Chattopadhyay PK, Chow S, Christakou E, Čičin-Šain L, Clerici M, Colombo FS, Cook L, Cooke A, Cooper AM, Corbett AJ, Cosma A, Cosmi L, Coulie PG, Cumano A, Cvetkovic L, Dang VD, Dang-Heine C, Davey MS, Davies D, De Biasi S, Del Zotto G, Dela Cruz GV, Delacher M, Della Bella S, Dellabona P, Deniz G, Dessing M, Di Santo JP, Diefenbach A, Dieli F, Dolf A, Dörner T, Dress RJ, Dudziak D, Dustin M, Dutertre CA, Ebner F, Eckle SBG, Edinger M, Eede P, Ehrhardt GRA, Eich M, Engel P, Engelhardt B, Erdei A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) Eur J Immunol. 2019 Oct;49(10):1457-1973.
7: Compton SJ, Jones CG. Mechanism of dye response and interference in the Bradford protein assay Anal Biochem. 1985 Dec;151(2):369-74.
8: Kielkopf CL, Bauer W, Urbatsch IL. Methods for Measuring the Concentrations of Proteins Cold Spring Harb Protoc. 2020 Apr 1;2020(4):102277.
9: Noble JE. Quantification of protein concentration using UV absorbance and Coomassie dyes Methods Enzymol. 2014;536:17-26.
10: Simonian MH. Spectrophotometric determination of protein concentration Curr Protoc Cell Biol. 2002 Aug;Appendix 3:Appendix 3B.

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