β-Galactosidase CRISPR Activation Plasmid

Product Description

β-Galactosidase CRISPR Activation Plasmid
Catalog number: B2014904
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 µg
Molecular Weight or Concentration: N/A
Supplied as: Liquid
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: β-Galactosidase CRISPR Activation Plasmid
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Watzlawick H, Altenbuchner J. Multiple integration of the gene ganA into the Bacillus subtilis chromosome for enhanced β-galactosidase production using the CRISPR/Cas9 system AMB Express. 2019 Sep 30;9(1):158.
2: Szentes S, Zsibrita N, Koncz M, Zsigmond E, Salamon P, Pletl Z, Kiss A. I-Block: a simple Escherichia coli-based assay for studying sequence-specific DNA binding of proteins Nucleic Acids Res. 2020 Mar 18;48(5):e28.
3: Manica A, Zebec Z, Teichmann D, Schleper C. In vivo activity of CRISPR-mediated virus defence in a hyperthermophilic archaeon Mol Microbiol. 2011 Apr;80(2):481-91.
4: Lau CH, Bolt EL. Integration of diverse DNA substrates by a casposase can be targeted to R-loops in vitro by its fusion to Cas9 Biosci Rep. 2021 Jan 29;41(1):BSR20203595.
5: Watzlawick H, Altenbuchner J. Multiple integration of the gene ganA into the Bacillus subtilis chromosome for enhanced β-galactosidase production using the CRISPR/Cas9 system AMB Express. 2019 Sep 30;9(1):158.
6: Tang Z, Hegde S, Zhao J, Zhu S, Johnson KA, Lorson CL, Wang J. CRISPR-Mediated Enzyme Fragment Complementation Assay for Quantification of the Stability of Splice Isoforms Chembiochem. 2022 May 4;23(9):e202200012.
7: Liu S, Feng Y, Huang Y, Jiang X, Tang C, Tang F, Zeng C, Liu L. A GM1 gangliosidosis mutant mouse model exhibits activated microglia and disturbed autophagy Exp Biol Med (Maywood). 2021 Jun;246(11):1330-1341.
8: Kaneski CR, Hanover JA, Schueler Hoffman UH. Generation of GLA-knockout human embryonic stem cell lines to model peripheral neuropathy in Fabry disease Mol Genet Metab Rep. 2022 Aug 27;33:100914.
9: de Malmanche H, Marcellin E, Reid S. Knockout of Sf-Caspase-1 generates apoptosis-resistant Sf9 cell lines: Implications for baculovirus expression Biotechnol J. 2022 Jul;17(7):e2100532.
10: Zhao R, Kaakati R, Liu X, Xu L, Lee AK, Bachelder R, Li CY, Hollenbeck ST. CRISPR/Cas9-Mediated BRCA1 Knockdown Adipose Stem Cells Promote Breast Cancer Progression Plast Reconstr Surg. 2019 Mar;143(3):747-756.

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