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A new interesting article has been published in J Glob Antimicrob Resist. 2019 Dec; 19:8-13. doi: 10.1016/j.jgar.2019.04.015. Epub 2019 Apr 30. and titled:

Evaluation of the AID AmpC line probe assay for molecular detection of AmpC-producing Enterobacterales.

Authors of this article are:

Wagner K, Mancini S, Ritter C, Böttger EC, Keller PM.

A summary of the article is shown below:

OBJECTIVES: In this study, the commercially available AID AmpC line probe assay (LPA) was evaluated for detection of plasmid-mediatedblaAmpC β-lactamase genes in Enterobacterales as well as chromosomal mutations in the blaAmpC promoter/attenuator regions in Escherichia coli.METHODS: Accuracy of the AID AmpC probes was assessed using Enterobacterales clinical isolates harbouring diverse plasmid-mediated AmpC enzymes (ACC, ACT, DHA, FOX, CMY and MOX) and E. coli clinical isolates with mutations in the chromosomal blaAmpC promoter/attenuator regions. The diagnostic performance of the AID AmpC LPA for blaAmpC detection directly from clinical specimens was determined using 99 clinical urine specimens with bacterial cell counts >105CFU/mL and the results were compared with culture-based phenotypic drug susceptibility testing (DST).RESULTS: Detection of blaAmpC genes in Enterobacterales clinical isolates showed 100% congruence with phenotypic DST results. The AID AmpC LPA showed 100% specificity [95% confidence interval (CI) 96-100%] and 100% sensitivity (95% CI 75-100%) for detection of plasmid-meditated blaAmpC and E. coli genomic blaAmpC promoter/attenuator mutations directly from clinical urine specimens. The AID AmpC LPA detected three AmpC-producers in urine specimens with bacterial cell counts >105CFU/mL that were missed by culture-based phenotypic DST, thereby displaying higher diagnostic sensitivity.CONCLUSION: The AID AmpC LPA is an accurate, sensitive and easy-to-use test that can be readily implemented in any diagnostic laboratory for molecular detection of blaAmpC genes in Enterobacterales.Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.

Check out the article’s website on Pubmed for more information:

[link-preview url=https://www.ncbi.nlm.nih.gov/pubmed/31051288 forceshot=true]

This article is a good source of information and a good way to become familiar with topics such as: AmpC β-lactamase; Antimicrobial resistance; Enterobacterales; Molecular detection; bla(AmpC).

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