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A new interesting article has been published in Talanta. 2019 Dec 1; 205:120104. doi: 10.1016/j.talanta.2019.06.104. Epub 2019 Jun 29. and titled:

Dried blood spot N-glycome analysis by MALDI mass spectrometry.

Authors of this article are:

Vreeker GCM, Bladergroen MR, Nicolardi S, Mesker WE, Tollenaar RAEM, van der Burgt YEM, Wuhrer M.

A summary of the article is shown below:

Body fluid N-glycome analysis as well as glyco-proteoform profiling of existing protein biomarkers potentially provides a stratification layer additional to quantitative, diagnostic protein levels. For clinical omics applications, the collection of a dried blood spot (DBS) is increasingly pursued as an alternative to sampling milliliters of peripheral blood. Here we evaluate DBS cards as a blood collection strategy for protein N-glycosylation analysis aiming for high-throughput clinical applications. A protocol for facile N-glycosylation profiling from DBS is developed that includes sialic acid linkage differentiation. This protocol is based on a previously established total plasma N-glycome mass spectrometry (MS) method, with adjustments for the analysis of DBS specimens. After DBS-punching and protein solubilization N-glycans are released, followed by chemical derivatization of sialic acids and MS-measurement of N-glycan profiles. With this method, more than 80 different glycan structures are identified from a DBS, with RSDs below 10% for the ten most abundant glycans. N-glycan profiles of finger-tip blood and venous blood are compared and short-term stability of DBS is demonstrated. This method for fast N-glycosylation profiling of DBS provides a minimally invasive alternative to conventional serum and plasma protein N-glycosylation workflows. With simplified blood sampling this DBS approach has vast potential for clinical glycomics applications.Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Check out the article’s website on Pubmed for more information:

[link-preview url=https://www.ncbi.nlm.nih.gov/pubmed/31450448 forceshot=true]

This article is a good source of information and a good way to become familiar with topics such as: Dried Blood Spot Testing; Glycomics; Humans; Polysaccharides; Sialic Acids; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.

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