WST-8 (Highly Pure)


Catalog Number: B2012549 (5 mg)
WST-8 (Highly Pure) is a high quality water-soluble tetrazolium salt used for assessing cell metabolic activity. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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WST-8 (Highly Pure)
Catalog number: B2012549
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 600.5 g/mol
Supplied as: Lyophilized Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: Water-Soluble Tetrazolium 8, 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium, inner salt, monosodium salt
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Präbst K, Engelhardt H, Ringgeler S, Hübner H. Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin Methods Mol Biol. 2017;1601:1-17.
2: Chamchoy K, Pakotiprapha D, Pumirat P, Leartsakulpanich U, Boonyuen U. Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays BMC Biochem. 2019 Apr 8;20(1):4.
3: Chen YH, Wu CP, Lin HL, Liaw RB, Lai YY, Wu MC, Chen LR, Jason Tsai PS. Tetrazolium Salt WST-8 as a Novel and Reliable Chromogenic Indicator for the Assessment of Boar Semen Quality Animals (Basel). 2020 Dec 4;10(12):2293.
4: Lutter AH, Scholka J, Richter H, Anderer U. Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures Clin Hemorheol Microcirc. 2017;67(3-4):327-342.
5: Stoddart MJ. WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells Methods Mol Biol. 2011;740:21-5.
6: Tiwari K, Wavdhane M, Haque S, Govender T, Kruger HG, Mishra MK, Chandra R, Tiwari D. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line Pharm Biol. 2015 Jun;53(6):849-54.
7: Chamchoy K, Praoparotai A, Pakparnich P, Sudsumrit S, Swangsri T, Chamnanchanunt S, Songdej D, Imwong M, Boonyuen U. The integrity and stability of specimens under different storage conditions for glucose-6-phosphate dehydrogenase deficiency screening using WST-8 Acta Trop. 2021 May;217:105864.
8: Kawano M, Hokazono E, Osawa S, Sato S, Tateishi T, Manabe M, Matsui H, Kayamori Y. A novel assay for triglycerides using glycerol dehydrogenase and a water-soluble formazan dye, WST-8 Ann Clin Biochem. 2019 Jul;56(4):442-449.
9: Tsukatani T, Suenaga H, Shiga M, Noguchi K, Ishiyama M, Ezoe T, Matsumoto K. Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria J Microbiol Methods. 2012 Sep;90(3):160-6.
10: Xie L, Dai Z, Pang C, Lin D, Zheng M. Cellular glucose metabolism is essential for the reduction of cell-impermeable water-soluble tetrazolium (WST) dyes Int J Biol Sci. 2018 Sep 7;14(11):1535-1544.

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