Urease Buffer Reagent

Description

Urease Buffer Reagent
Catalog number: B2013529
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 vial
Molecular Weight or Concentration: 545 kDa
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: 2-8°C
Keywords: Urea colorimetric determination assay reagent
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Lapierre FM, Bolz I, Büchs J, Huber R. Developing a fluorometric urease activity microplate assay suitable for automated microbioreactor experiments Front Bioeng Biotechnol. 2022 Sep 14;10:936759.
2: Amani A, Kalajahi ST, Yazdian F, Mirzababaei S, Rashedi H, Faramarzi MA, Vahidi M. Immobilization of urease enzyme on chitosan/polyvinyl alcohol electrospun nanofibers Biotechnol Prog. 2022 Sep;38(5):e3282.
3: Swanberg SL, Masover GK, Hayflick L. Some characteristics of Ureaplasma urealyticum. Urease activity in a simple buffer: effect of metal ions and sulphydryl inhibitors J Gen Microbiol. 1978 Oct;108(2):221-5.
4: Akkas T, Zakharyuta A, Taralp A, Ow-Yang CW. Cross-linked enzyme lyophilisates (CLELs) of urease: A new method to immobilize ureases Enzyme Microb Technol. 2020 Jan;132:109390.
5: Muddana HS, Sengupta S, Mallouk TE, Sen A, Butler PJ. Substrate catalysis enhances single-enzyme diffusion J Am Chem Soc. 2010 Feb 24;132(7):2110-1.
6: Kot M, Zaborska W. Irreversible inhibition of jack bean urease by pyrocatechol J Enzyme Inhib Med Chem. 2003 Oct;18(5):413-7.
7: Mo ZZ, Wang XF, Zhang X, Su JY, Chen HM, Liu YH, Zhang ZB, Xie JH, Su ZR. Andrographolide sodium bisulphite-induced inactivation of urease: inhibitory potency, kinetics and mechanism BMC Complement Altern Med. 2015 Jul 16;15:238.
8: Leung WK, Sung JJ, Siu KL, Chan FK, Ling TK, Cheng AF. False-negative biopsy urease test in bleeding ulcers caused by the buffering effects of blood Am J Gastroenterol. 1998 Oct;93(10):1914-8.
9: Zaborska W, Krajewska B, Kot M, Karcz W. Quinone-induced inhibition of urease: elucidation of its mechanisms by probing thiol groups of the enzyme Bioorg Chem. 2007 Jun;35(3):233-42.
10: Kot M, Bicz A. Inactivation of jack bean urease by N-ethylmaleimide: pH dependence, reversibility and thiols influence J Enzyme Inhib Med Chem. 2008 Aug;23(4):514-20.

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