Succinyl-L-alanyl-L-prolyl-L-alanine p-nitroanilide

Description

Succinyl-L-alanyl-L-prolyl-L-alanine p-nitroanilide
Catalog number: MDP0157
CAS Number: 396717-86-5
Sequence: Succinyl-L-alanyl-L-prolyl-L-alanine p-nitroanilide
Amount: 20 mg
Molecular Weight: 477.2 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20 °C
WARNING: For in vitro Research Use Only. NOT for use on humans or animals.
Grade: Biotechnology grade. All products are highly pure.

References:
1: Fukal L, Kasafírek E. N-succinyl-alanyl-methionyl-S-benzylcysteine p-nitroanilide as a sensitive substrate for assaying activity of cysteine proteinases J Biochem Biophys Methods. 1988 Aug;16(4):323-5.
2: Hayakawa T, Naruse S, Kitagawa M, Kondo T. [Elastase] Nihon Rinsho. 1995 May;53(5):1192-7.
3: Kramps JA, van Twisk C, van der Linden AC. L-Pyroglutamyl-L-prolyl-L-valine-p-nitroanilide, a highly specific substrate for granulocyte elastase Scand J Clin Lab Invest. 1983 Sep;43(5):427-32.
4: Sharma SK, Castellino FJ. The chemical synthesis of the chromogenic substrates, H-D-Val-L-Leu-L-Lys-p-nitroanilide (S2251) and H-D-Ile-L-Pro-L-ARG-p-nitroanilide (S2288) Thromb Res. 1990 Jan 1;57(1):127-38.
5: Filippova IYu, Lysogorskaya EN, Oksenoit ES, Rudenskaya GN, Stepanov VM. L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide–a chromogenic substrate for thiol proteinase assay Anal Biochem. 1984 Dec;143(2):293-7.
6: Ishida M, Ogawa M, Mori T, Mega T. Determination and characterization of succinyl tri-alanine p-nitroanilide hydrolyzing metalloendopeptidase in serum Enzyme. 1987;37(4):202-7.
7: Pastor I, Vilaseca E, Madurga S, Garcés JL, Cascante M, Mas F. Effect of crowding by dextrans on the hydrolysis of N-Succinyl-L-phenyl-Ala-p-nitroanilide catalyzed by α-chymotrypsin J Phys Chem B. 2011 Feb 10;115(5):1115-21.
8: Held C, Stolzke T, Knierbein M, Jaworek MW, Luong TQ, Winter R, Sadowski G. Cosolvent and pressure effects on enzyme-catalysed hydrolysis reactions Biophys Chem. 2019 Sep;252:106209.
9: Tanaka H, Shimazu T, Sugimoto H, Yoshioka T, Sugimoto T. A sensitive and specific assay for granulocyte elastase in inflammatory tissue fluid using L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide Clin Chim Acta. 1990 Feb 28;187(2):173-80.
10: Jacquot-Armand Y, Krebs G. Mise en evidence de la formation d’un complexe ternaire entre trypsine, alpha(2)-macroglobuline et inhibiteur basique de pancreas FEBS Lett. 1969 Jul;4(1):21-24.

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