Streptavidin (from streptomyces avidinii)

Product Description

Streptavidin
Catalog number: B2010214
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 10 mg
pH: na
Supplied as: Ready-to-use
Appearance: Lyophilized Powder
Applications: highly purified Streptavidin protein, a tetrameric protein with a very high affinity to biotin (Kd=10E-15 M).
Storage: -20C
Keywords: streptavidin from streptomyces avidinii
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Bayer EA, Kulik T, Adar R, Wilchek M. Close similarity among streptavidin-
like, biotin-binding proteins from Streptomyces. Biochim Biophys Acta. 1995 Jul
25;1263(1):60-6.

2: Weber PC, Cox MJ, Salemme FR, Ohlendorf DH. Crystallographic data for
Streptomyces avidinii streptavidin. J Biol Chem. 1987 Sep 15;262(26):12728-9.

3: Noda S, Matsumoto T, Tanaka T, Kondo A. Secretory production of tetrameric
native full-length streptavidin with thermostability using Streptomyces lividans
as a host. Microb Cell Fact. 2015 Jan 13;14:5.

4: Verougstraete N, Berth M, Vaneechoutte M, Delanghe J, Callewaert N.
Interference of anti-streptavidin antibodies in immunoassays: a very rare
phenomenon or a more common finding? Clin Chem Lab Med. 2020 Sep
25;58(10):1673-1680.

5: Wetzel D, Müller JM, Flaschel E, Friehs K, Risse JM. Fed-batch production and
secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors
and bioprocess development. J Biotechnol. 2016 May 10;225:3-9.

6: Agrawal N, Lehtonen SI, Uusi-Mäkelä M, Jain P, Viitala S, Määttä JAE,
Kähkönen N, Azizi L, Riihimäki TA, Kulomaa MS, Johnson MS, Hytönen VP, Airenne
TT. Molecular features of steroid-binding antidins and their use for assaying
serum progesterone. PLoS One. 2019 Feb 20;14(2):e0212339.

7: Hofmann K, Wood SW, Brinton CC, Montibeller JA, Finn FM. Iminobiotin affinity
columns and their application to retrieval of strept avidin. Proc Natl Acad Sci U
S A. 1980 Aug;77(8):4666-8.

8: Furukawa H, Tanino T, Fukuda H, Kondo A. Development of novel yeast cell
surface display system for homo-oligomeric protein by coexpression of native and
anchored subunits. Biotechnol Prog. 2006 Jul-Aug;22(4):994-7.

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