Recombinant endo-β-N-Acetylglucosaminidase

Description

Recombinant endo-β-N-Acetylglucosaminidase
Catalog number: B2011283
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 ug
Molecular Weight or Concentration: N/A
Supplied as: Lyophilized
Applications: molecular tool for various biochemical applications
Storage: -80 °C
Keywords: Endo-M, Endo-M Recombinant: from Mucor hiemalis expressed in Candida boidinii [Purity: single band by SDS-PAGE(85KDa)]
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Cohen JL, Karav S, Barile D, de Moura Bell JMLN. Immobilization of an Endo-β-N-acetylglucosaminidase for the Release of Bioactive N-glycans Catalysts. 2018 Jul;8(7):278.
2: Maeda M, Okamoto N, Araki N, Kimura Y. Purification, Characterization, and Gene Expression of Rice Endo-β-N-Acetylglucosaminidase, Endo-Os Front Plant Sci. 2021 Aug 10;12:647684.
3: Li L, Hu S, Gu T, Lü C, Liu Y, Liu H, Gu J, Zhao G. [Cloning, expression and characterization of a new endo-β-N-acetylglucosaminidase from Streptomyces alfalfae] Sheng Wu Gong Cheng Xue Bao. 2020 May 25;36(5):932-941.
4: Nandakumar KS, Collin M, Happonen KE, Lundström SL, Croxford AM, Xu B, Zubarev RA, Rowley MJ, Blom AM, Kjellman C, Holmdahl R. Streptococcal Endo-β-N-Acetylglucosaminidase Suppresses Antibody-Mediated Inflammation In Vivo Front Immunol. 2018 Jul 16;9:1623.
5: Mamedov T, Cicek K, Gulec B, Ungor R, Hasanova G. In vivo production of non-glycosylated recombinant proteins in Nicotiana benthamiana plants by co-expression with Endo-β-N-acetylglucosaminidase H (Endo H) of Streptomyces plicatus PLoS One. 2017 Aug 21;12(8):e0183589.
6: Eshima Y, Higuchi Y, Kinoshita T, Nakakita S, Takegawa K. Transglycosylation Activity of Glycosynthase Mutants of Endo-β-N-Acetylglucosaminidase from Coprinopsis cinerea PLoS One. 2015 Jul 21;10(7):e0132859.
7: Fujita K, Kobayashi K, Iwamatsu A, Takeuchi M, Kumagai H, Yamamoto K. Molecular cloning of Mucor hiemalis endo-beta-N-acetylglucosaminidase and some properties of the recombinant enzyme Arch Biochem Biophys. 2004 Dec 1;432(1):41-9.
8: Murakami S, Takaoka Y, Ashida H, Yamamoto K, Narimatsu H, Chiba Y. Identification and characterization of endo-β-N-acetylglucosaminidase from methylotrophic yeast Ogataea minuta Glycobiology. 2013 Jun;23(6):736-44.
9: Wang F, Wang X, Yu X, Fu L, Liu Y, Ma L, Zhai C. High-level expression of endo-β-N-acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and its application for the deglycosylation of glycoproteins PLoS One. 2015 Mar 17;10(3):e0120458.
10: Takashima S, Kurogochi M, Osumi K, Sugawara SI, Mizuno M, Takada Y, Amano J, Matsuda A. Novel endo-β-N-acetylglucosaminidases from Tannerella species hydrolyze multibranched complex-type N-glycans with different specificities Glycobiology. 2020 Oct 21;30(11):923-934.

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