Protein A Conjugate to Cy3

Description

Protein A Conjugate to Cy3
Catalog number: B2013425
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 mg
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: Protein A-Cy3
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Nguyen B, Ciuba MA, Kozlov AG, Levitus M, Lohman TM. Protein Environment and DNA Orientation Affect Protein-Induced Cy3 Fluorescence Enhancement Biophys J. 2019 Jul 9;117(1):66-73.
2: Nguyen B, Sokoloski J, Galletto R, Elson EL, Wold MS, Lohman TM. Diffusion of human replication protein A along single-stranded DNA J Mol Biol. 2014 Sep 23;426(19):3246-3261.
3: Hedglin M, Benkovic SJ. Replication Protein A Prohibits Diffusion of the PCNA Sliding Clamp along Single-Stranded DNA Biochemistry. 2017 Apr 4;56(13):1824-1835.
4: Koukiekolo R, Jakubek ZJ, Cheng J, Sagan SM, Pezacki JP. Studies of a viral suppressor of RNA silencing p19-CFP fusion protein: a FRET-based probe for sensing double-stranded fluorophore tagged small RNAs Biophys Chem. 2009 Aug;143(3):166-9.
5: McCarthy ME, Anglin CM, Peer HA, Boleman SA, Klaubert SR, Birtwistle MR. Protocol for Creating Antibodies with Complex Fluorescence Spectra Bioconjug Chem. 2021 Jun 16;32(6):1156-1166.
6: Schneider JR, Carias AM, Bastian AR, Cianci GC, Kiser PF, Veazey RS, Hope TJ. Long-term direct visualization of passively transferred fluorophore-conjugated antibodies J Immunol Methods. 2017 Nov;450:66-72.
7: Ren X, Xue Q, Wen L, Li X, Wang H. Fluorescence-enhanced p19 proteins-conjugated single quantum dot with multiplex antenna for one-step, specific and sensitive miRNAs detection Anal Chim Acta. 2019 Apr 11;1053:114-121.
8: Naganbabu M, Perkins LA, Wang Y, Kurish J, Schmidt BF, Bruchez MP. Multiexcitation Fluorogenic Labeling of Surface, Intracellular, and Total Protein Pools in Living Cells Bioconjug Chem. 2016 Jun 15;27(6):1525-31.
9: Portet A, Galinier R, Lassalle D, Faille A, Gourbal B, Duval D. Hemocyte siRNA uptake is increased by 5′ cholesterol-TEG addition in Biomphalaria glabrata, snail vector of schistosome PeerJ. 2021 Feb 23;9:e10895.
10: Grimme JM, Spies M. FRET-based assays to monitor DNA binding and annealing by Rad52 recombination mediator protein Methods Mol Biol. 2011;745:463-83.

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