Phthaldialdehyde Reagent (Highly Pure)


Catalog Number: B2012906 (25 mL)
Phthaldialdehyde Reagent (Highly Pure) is a high quality reagent used for the derivatization plasma samples prior to high performance liquid chromatography. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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Phthaldialdehyde Reagent (Highly Pure)
Catalog number: B2012906
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 25 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: 2-8°C
Keywords: OPA
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Lee KS, Drescher DG. Fluorometric amino-acid analysis with o-phthaldialdehyde (OPA) Int J Biochem. 1978;9(7):457-67.
2: Chen RF, Scott C, Trepman E. Fluorescence properties of o-phthaldialdehyde derivatives of amino acids Biochim Biophys Acta. 1979 Feb 26;576(2):440-55.
3: Levinson SS. Kinetic centrifugal analyzer and manual determination of serum urea nitrogen, with use of o-phthaldialdehyde reagent Clin Chem. 1978 Dec;24(12):2199-202.
4: Flanagan J, FitzGerald RJ. Characterisation and quantification of the reaction(s) catalysed by transglutaminase using the o-phthaldialdehyde reagent Nahrung. 2003 Jun;47(3):207-12.
5: Kutlán D, Presits P, Molnár-Perl I. Behavior and characteristics of amine derivatives obtained with o-phthaldialdehyde/3-mercaptopropionic acid and with o-phthaldialdehyde/N-acetyl-L-cysteine reagents J Chromatogr A. 2002 Mar 8;949(1-2):235-48.
6: Lee KS, Drescher DG. Derivatization of cysteine and cystine for fluorescence amino acid analysis with the o-phthaldialdehyde/2-mercaptoethanol reagent J Biol Chem. 1979 Jul 25;254(14):6248-51.
7: Huang CC, Tseng WL. Highly selective detection of histidine using o-phthaldialdehyde derivatization after the removal of aminothiols through Tween 20-capped gold nanoparticles Analyst. 2009 Aug;134(8):1699-705.
8: Tivesten A, Folestad S. Chiral o-phthaldialdehyde reagents for fluorogenic on-column labeling of D- and L-amino acids in micellar electrokinetic chromatography Electrophoresis. 1997 Jun;18(6):970-7.
9: Lochmann D, Stadlhofer S, Weyermann J, Zimmer A. New protamine quantification method in microtiter plates using o-phthaldialdehyde/N-acetyl-L-cysteine reagent Int J Pharm. 2004 Sep 28;283(1-2):11-7.
10: Molnár-Perl I. Derivatization and chromatographic behavior of the o-phthaldialdehyde amino acid derivatives obtained with various SH-group-containing additives J Chromatogr A. 2001 Apr 13;913(1-2):283-302.

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