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Loading Buffer for RNA

Original price was: $795.00.Current price is: $395.00.

Catalog Number: B2027175 (2 mL)
This RNA loading buffer is a pre-mixed solution designed for the preparation and enzymatic inactivation of RNA samples prior to agarose or polyacrylamide gel electrophoresis. Formulated with high-purity denaturing agents like formamide or urea, it disrupts secondary RNA structures to ensure sharp, accurate band resolution during migration. It includes tracking dyes for visual monitoring of migration fronts and is certified RNase-free to prevent sample degradation during handling. Custom bulk amounts of this product are available upon request.

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Product Description

5/5 - (3 votes)

Loading Buffer for RNA
Catalog number: B2027175
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 2 mL
Molecular Weight or Concentration: NA
Supplied as: Solution
Storage: RT
Keywords: RNA Sample Loading Buffer, RNA loading dye

References:
1. Sambrook J, Russell DW. Agarose gel electrophoresis of RNA. Cold Spring Harbor Protocols. 2006;2006(1):pdb.prot4065.
2. Mansour MH, Wettenhall RE. Formamide-based loading buffers for the analysis of RNA by polyacrylamide gel electrophoresis. Analytical Biochemistry. 1998;255(1):161-163.
3. Rio DC, Ares M, Hannon GJ, Nilsen TW. Nondenaturing agarose gel electrophoresis of RNA. Cold Spring Harbor Protocols. 2010;2010(6):pdb.prot5445.
4. Masek T, Vopalensky V, Suchomelova P, Pospisek M. Denaturing RNA electrophoresis in TAE agarose gels. Analytical Biochemistry. 2005;336(1):46-50.
5. Goda SK, Minton NP. A simple and rapid method for the preparation of total RNA from various sources. Nucleic Acids Research. 1995;23(16):3357-3358.
6. Lehrach H, Diamond D, Wozney JM, Boedtker H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977;16(21):4743-4751.
7. Kroczek RA, Siebert E. Optimization of RNA blot hybridization by using formamide-containing loading buffers. Analytical Biochemistry. 1990;184(1):90-95.
8. Thomas PS. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences. 1980;77(9):5201-5205.
9. Pellé R, Murphy NB. Northern hybridization: rapid transfer of RNA from agarose gels to nylon membranes. Nucleic Acids Research. 1993;21(11):2783-2784.
10. Rosen KM, Villa-Komaroff L. An improved method for isolation of RNA from cells of the central nervous system. Journal of Neuroscience Research. 1990;26(4):519-525.
Loading Buffer for RNA

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Additional Information

Weight 0.15 oz
Dimensions 2 × 0.5 × 0.5 in
5/5 - (3 votes)

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