L-Isoleucine (Biotechnology Grade, High Purity)

$150.00

Catalog Number: B2010174 (10 g)
L-Isoleucine is a high quality research product used as multipurpose amino acid for protein synthesis, reagent stabilization, preparation of culture media and more. Custom bulk orders of this product are available upon request.

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SKU: B2010174 Category:

Description

L-Isoleucine
Catalog number: B2010174
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 10 g
pH: na
Supplied as: Ready-to-use
Appearance: White Powder
Applications: multipurpose amino acid for protein synthesis, reagent stabilization, preparation of culture media and more.
Storage: Room temperature
Keywords: (2S,3S)-2-Amino-3-methylpentanoic acid
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Wang X. Strategy for improving L-isoleucine production efficiency in
Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2019
Mar;103(5):2101-2111.

2: Yin L, Shi F, Hu X, Chen C, Wang X. Increasing l-isoleucine production in
Corynebacterium glutamicum by overexpressing global regulator Lrp and two-
component export system BrnFE. J Appl Microbiol. 2013 May;114(5):1369-77.

3: Ma W, Wang J, Li Y, Yin L, Wang X. Poly(3-hydroxybutyrate-
co-3-hydroxyvalerate) co-produced with L-isoleucine in Corynebacterium
glutamicum WM001. Microb Cell Fact. 2018 Jun 15;17(1):93.

4: Ma W, Wang J, Li Y, Wang X. Cysteine synthase A overexpression in
Corynebacterium glutamicum enhances l-isoleucine production. Biotechnol Appl
Biochem. 2019 Jan;66(1):74-81.

5: Zhang C, Ma J, Li Z, Liang Y, Xu Q, Xie X, Chen N. A strategy for
L-isoleucine dioxygenase screening and 4-hydroxyisoleucine production by resting
cells. Bioengineered. 2018 Jan 1;9(1):72-79. doi: 10.1080/21655979.2017.1304872.
Epub 2017 Apr 28. Erratum in: Bioengineered. 2017 Nov 2;8(6):759.

6: Dong XY, Azzam MM, Zou XT. Effects of dietary L_isoleucine on laying
performance and immunomodulation of laying hens. Poult Sci. 2016 Oct
1;95(10):2297-305.

7: Lee KM, Kim SK, Lee YG, Park KH, Seo JH. Elimination of biosynthetic pathways
for l-valine and l-isoleucine in mitochondria enhances isobutanol production in
engineered Saccharomyces cerevisiae. Bioresour Technol. 2018 Nov;268:271-277.

8: Smirnov SV, Sokolov PM, Kotlyarova VA, Samsonova NN, Kodera T, Sugiyama M,
Torii T, Hibi M, Shimizu S, Yokozeki K, Ogawa J. A novel
l-isoleucine-4′-dioxygenase and l_isoleucine dihydroxylation cascade in Pantoea
ananatis. Microbiologyopen. 2013 Jun;2(3):471-81.

9: Huang S, Shi F. Directed evolution and site-specific mutagenesis of
L_isoleucine dioxygenase derived from Bacillus weihenstephanensis. Biotechnol
Lett. 2018 Aug;40(8):1227-1235.

10: Newmire DE, Rivas E, Deemer SE, Willoughby DS, Ben-Ezra V. The Impact of a
Large Bolus Dose of l_leucine and l-isoleucine on Enteroendocrine and Pancreatic
Hormones, and Glycemia in Healthy, Inactive Adults. Nutrients. 2019 Nov
4;11(11):2650.

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