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Fluorogenic Caspase 1 Substrate 3f

Original price was: $1,195.00.Current price is: $595.00.

Catalog Number: B2011406 (5 mg)
Fluorogenic Caspase 1 Substrate 3f is a high quality AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrate for the fluorimetric detection of caspase activities. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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Product Description

4.8/5 - (6 votes)

Fluorogenic Caspase 1 Substrate 3f
Catalog number: B2011406
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 838.8 g/mol
Supplied as: Lyophilized
Applications: molecular tool for various biochemical applications
Storage: -20 °C
Keywords: Caspase 1 (ICE) Substrate 3f, fluorogenic
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Boucher D, Chan A, Ross C, Schroder K. Quantifying Caspase-1 Activity in Murine Macrophages Methods Mol Biol. 2018;1725:163-176.
2: Qian L, Zhang CJ, Wu J, Yao SQ. Fused Bicyclic Caspase-1 Inhibitors Assembled by Copper-Free Strain-Promoted Alkyne-Azide Cycloaddition (SPAAC) Chemistry. 2017 Jan 5;23(2):360-369.
3: Rahman MA, Mitra S, Sarkar A, Wewers MD. Alpha 1-antitrypsin does not inhibit human monocyte caspase-1 PLoS One. 2015 Feb 6;10(2):e0117330.
4: Schumann RR, Belka C, Reuter D, Lamping N, Kirschning CJ, Weber JR, Pfeil D. Lipopolysaccharide activates caspase-1 (interleukin-1-converting enzyme) in cultured monocytic and endothelial cells Blood. 1998 Jan 15;91(2):577-84.
5: Kindermann M, Roschitzki-Voser H, Caglic D, Repnik U, Miniejew C, Mittl PR, Kosec G, Grütter MG, Turk B, Wendt KU. Selective and sensitive monitoring of caspase-1 activity by a novel bioluminescent activity-based probe Chem Biol. 2010 Sep 24;17(9):999-1007.
6: Ranganathan R, Lenti G, Tassone NM, Scannell BJ, Southern CA, Karver CE. Design and application of a fluorogenic assay for monitoring inflammatory caspase activity Anal Biochem. 2018 Feb 15;543:1-7.
7: Fahy RJ, Doseff AI, Wewers MD. Spontaneous human monocyte apoptosis utilizes a caspase-3-dependent pathway that is blocked by endotoxin and is independent of caspase-1 J Immunol. 1999 Aug 15;163(4):1755-62.
8: Karahashi H, Amano F. Changes of caspase activities involved in apoptosis of a macrophage-like cell line J774.1/JA-4 treated with lipopolysaccharide (LPS) and cycloheximide Biol Pharm Bull. 2000 Feb;23(2):140-4.
9: Li M, Ona VO, Chen M, Kaul M, Tenneti L, Zhang X, Stieg PE, Lipton SA, Friedlander RM. Functional role and therapeutic implications of neuronal caspase-1 and -3 in a mouse model of traumatic spinal cord injury Neuroscience. 2000;99(2):333-42.
10: Roy S, Sharom JR, Houde C, Loisel TP, Vaillancourt JP, Shao W, Saleh M, Nicholson DW. Confinement of caspase-12 proteolytic activity to autoprocessing Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4133-8.

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Additional Information

Weight 0.15 oz
Dimensions 2 × 0.5 × 0.5 in
4.8/5 - (6 votes)

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