Fluorescent Thrombin Substrate

Product Description

Fluorescent Thrombin Substrate
Catalog number: B2011314
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 629.6 g/mol
Supplied as: Lyophilized
Applications: molecular tool for various biochemical applications
Storage: -20 °C
Keywords: Thrombin Substrate, fluorescent (H-D-Phe-Pro-Arg-AFC)
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Ito Y, Liu LS, Imanishi Y. Interaction of thrombin with synthetic fluorescent substrate immobilized on polymer membrane Biomaterials. 1992;13(6):375-81.
2: Leung K. Cy7-Tyr-d-Glu-Cys-Hyp-Tyr(3-Cl)-Gly-Leu-Cys-Tyr-Ile-Gln-NH(2) 2013 Apr 27 [updated 2013 May 30]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013.
3: Gasa N, Meiring M. Microparticles: a link to increased thrombin generation Blood Coagul Fibrinolysis. 2021 Apr 1;32(3):204-208.
4: Hemker HC, Kremers R. Data management in thrombin generation Thromb Res. 2013 Jan;131(1):3-11.
5: Biscombe CJC, Dower SK, Muir IL, Harvie DJE. Modeling Thrombin Generation in Plasma under Diffusion and Flow Biophys J. 2020 Jul 7;119(1):162-181.
6: Zhang Y, Li B, Jin Y. Label-free fluorescent detection of thrombin using G-quadruplex-based DNAzyme as sensing platform Analyst. 2011 Aug 21;136(16):3268-73.
7: Panizzi P, Friedrich R, Fuentes-Prior P, Richter K, Bock PE, Bode W. Fibrinogen substrate recognition by staphylocoagulase.(pro)thrombin complexes J Biol Chem. 2006 Jan 13;281(2):1179-87.
8: Wang C, Zhao Q. A competitive thrombin-linked aptamer assay for small molecule: aflatoxin B(1) Anal Bioanal Chem. 2019 Oct;411(25):6637-6644.
9: Itsekson Hayosh Z, Abu Bandora E, Shelestovich N, Nulman M, Bakon M, Yaniv G, Khaitovitch B, Balan S, Gerasimova A, Drori T, Mausbach S, Schwammenthal Y, Afek A, Chapman J, Shavit Stein E, Orion D. In-thrombus thrombin secretion: a new diagnostic marker of atrial fibrillation in cryptogenic stroke J Neurointerv Surg. 2021 Sep;13(9):799-804.
10: Chen F, Zhao Z, Zhou J, Lu Y, Essex DW, Wu Y. Protein disulfide isomerase enhances tissue factor-dependent thrombin generation Biochem Biophys Res Commun. 2018 Jun 18;501(1):172-177.

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