E. coli Maltose Binding Protein (MBP)

Product Description

E. coli Maltose Binding Protein (MBP)
Catalog number: B2015197
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 ug
Molecular Weight or Concentration: 42 kDa (387 aa)
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -20° C
Keywords: Maltodextrin-binding protein, malE, malJ, ECK4026, JW3994, Maltodextrin-binding protein, Maltose-binding periplasmic protein, MBP, MMBP, Maltose ABC transporter periplasmic protein
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Lebendiker M, Danieli T. Purification of proteins fused to maltose-binding protein Methods Mol Biol. 2011;681:281-93.
2: Raran-Kurussi S, Cherry S, Zhang D, Waugh DS. Removal of Affinity Tags with TEV Protease Methods Mol Biol. 2017;1586:221-230.
3: Schubeis T, Stanek J, Pintacuda G. Backbone assignment of crystalline E. coli maltose binding protein Biomol NMR Assign. 2021 Oct;15(2):317-322.
4: Liu G, Zhang Y, Zhang N, Ni W, Jie J, Jiang L, Tai G. Escherichia coli maltose-binding protein (MBP) activates mouse Th1 through TLR2-mediated MyD88-dependent pathway and TLR4-mediated TRIF-dependent pathway Int Immunopharmacol. 2017 Sep;50:338-344.
5: Raran-Kurussi S, Waugh DS. Expression and Purification of Recombinant Proteins in Escherichia coli with a His(6) or Dual His(6)-MBP Tag Methods Mol Biol. 2017;1607:1-15.
6: Li Y, Sousa R. Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation Protein Expr Purif. 2012 Mar;82(1):162-7.
7: Lee SB, Park SK, Kim YS. Maltose binding protein-fusion enhances the bioactivity of truncated forms of pig myostatin propeptide produced in E. coli PLoS One. 2017 Apr 3;12(4):e0174956.
8: Kobashigawa Y, Namikawa M, Sekiguchi M, Inada Y, Yamauchi S, Kimoto Y, Okazaki K, Toyota Y, Sato T, Morioka H. Expression, Purification and Characterization of CAR/NCOA-1 Tethered Protein in E. coli Using Maltose-Binding Protein Fusion Tag and Gelatinized Corn Starch Biol Pharm Bull. 2021;44(1):125-130.
9: Lebendiker M, Danieli T. Purification of Proteins Fused to Maltose-Binding Protein Methods Mol Biol. 2017;1485:257-273.
10: Lénon M, Ke N, Ren G, Meuser ME, Loll PJ, Riggs P, Berkmen M. A useful epitope tag derived from maltose binding protein Protein Sci. 2021 Jun;30(6):1235-1246.

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