DAPI (Highly Pure)

Product Description

DAPI (Highly Pure)
Catalog number: B2010984
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 350.3 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical studies
Storage: -20C
Keywords: 4′,6-Diamidino-2-phenylindole Dihydrochloride, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride, 6-Amidino-2-(4-amidinophenyl)indole Dihydrochloride, 4′,6-diamidino-2-phenylindole
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Kapuscinski J. DAPI: a DNA-specific fluorescent probe Biotech Histochem. 1995 Sep;70(5):220-33.
2: Chazotte B. Labeling nuclear DNA using DAPI Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5556.
3: Rana R, Chang Q, Bassan J, Chow S, Hedley D, Nitz M. An Iodinated DAPI-Based Reagent for Mass Cytometry Chembiochem. 2021 Feb 2;22(3):532-538.
4: Omelon S, Georgiou J, Habraken W. A cautionary (spectral) tail: red-shifted fluorescence by DAPI-DAPI_ interactions Biochem Soc Trans. 2016 Feb;44(1):46-9.
5: Mora AK , Khan S , Patro BS , Nath S . Is DAPI_ assay of cellular nucleic acid reliable in the presence of protein aggregates? Chem Commun (Camb). 2020 Nov 18;56(89):13844-13847.
6: Parameswaran P, Arora Y, Patidar R, Ranjan N. Bacterial rRNA A-site recognition by DAPI_: Signatures of intercalative binding Biophys Chem. 2021 Jul;274:106589.
7: Munyenyembe K, Timmons C, Weiner AKM, Katz LA, Yan Y. DAPI_ staining and DNA content estimation of nuclei in uncultivable microbial eukaryotes (Arcellinida and Ciliates) Eur J Protistol. 2021 Oct;81:125840.
8: Crowley LC, Marfell BJ, Waterhouse NJ. Analyzing Cell Death by Nuclear Staining with Hoechst 33342 Cold Spring Harb Protoc. 2016 Sep 1;2016(9).
9: Musetti R, Buxa SV. DAPI and Confocal Laser-Scanning Microscopy for In Vivo Imaging of Phytoplasmas Methods Mol Biol. 2019;1875:301-306.
10: Digby EM , Rana R , Nitz M , Beharry AA . DNA directed damage using a brominated DAPI derivative Chem Commun (Camb). 2019 Aug 15;55(67):9971-9974.

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