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CHES Buffer (Biochemistry Grade)

$175.00

Catalog Number: B2010065 (50 g)
CHES is a high quality research product used as mutipurpose buffer for various biochemical, molecular biology and molecular diagnostic applications. CHES is a Good′s buffer that has been used in protein stabilization, mannanase activity assay, DNA digestion and labeling of 2′-deoxyadenosine 3′-monophosphate and as a component of Wilson and Horne extraction buffer. Custom bulk orders of this product are available upon request.

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SKU: B2010065 Categories: ,

Description

CHES
Catalog number: B2010065
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 50 g
pH: na
Supplied as: Ready-to-use
Appearance: White Powder
Applications: mutipurpose buffer for various biochemical, molecular biology and molecular diagnostic applications. CHES is a Good′s buffers that has been used in protein stabilization, mannanase activity assay, DNA digestion and labeling of 2′-deoxyadenosine 3′-monophosphate and as a component of Wilson and Horne extraction buffer.
Storage: Room temperature
Keywords: 2-(Cyclohexylamino)ethanesulfonic acid
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Farsang E, Murisier A, Horváth K, Beck A, Kormány R, Guillarme D, Fekete S.
Tuning selectivity in cation-exchange chromatography applied for monoclonal
antibody separations, part 1: Alternative mobile phases and fine tuning of the
separation. J Pharm Biomed Anal. 2019 May 10;168:138-147.

2: Gäb J, John H, Melzer M, Blum MM. Stable adducts of nerve agents sarin, soman
and cyclosarin with TRIS, TES and related buffer compounds–characterization by
LC-ESI-MS/MS and NMR and implications for analytical chemistry. J Chromatogr B
Analyt Technol Biomed Life Sci. 2010 May 15;878(17-18):1382-90.

3: Kadam RU, Wilson IA. A small-molecule fragment that emulates binding of
receptor and broadly neutralizing antibodies to influenza A hemagglutinin. Proc
Natl Acad Sci U S A. 2018 Apr 17;115(16):4240-4245.

4: Lee Y, Youn HS, Lee JG, An JY, Park KR, Kang JY, Ryu YB, Jin MS, Park KH, Eom
SH. Crystal structure of the catalytic domain of Clostridium perfringens
neuraminidase in complex with a non-carbohydrate-based inhibitor,
2-(cyclohexylamino)ethanesulfonic acid. Biochem Biophys Res Commun. 2017 Apr
29;486(2):470-475.

5: Collins B, Stevens RC, Page R. Crystallization Optimum Solubility Screening:
using crystallization results to identify the optimal buffer for protein crystal
formation. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Dec 1;61(Pt
12):1035-8.

6: Lu Q, Collins GE. A fritless, EOF microchip pump for high pressure pumping of
aqueous and organic solvents. Lab Chip. 2009 Apr 7;9(7):954-60.

7: Sumitomo K, Mayumi K, Minamikawa H, Masuda M, Asahi T, Shimizu T, Ito K,
Yamaguchi Y. Buffers to suppress sodium dodecyl sulfate adsorption to
polyethylene oxide for protein separation on capillary polymer electrophoresis.
Electrophoresis. 2011 Feb;32(3-4):448-54.

8: Xu G, Potter JA, Russell RJ, Oggioni MR, Andrew PW, Taylor GL. Crystal
structure of the NanB sialidase from Streptococcus pneumoniae. J Mol Biol. 2008
Dec 12;384(2):436-49.

Products Related to CHES: Buffers & Solutions

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