bis-ANS (Highly Pure)


Catalog Number: B2012412 (5 mg)
bis-ANS (Highly Pure) is a high quality bis-ANS potassium salt used as a high-affinity non-covalent extrinsic fluorescent dye for protein conformation. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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bis-ANS (Highly Pure)
Catalog number: B2012412
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 672.9 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: 4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulfonic Acid
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Dobieżyńska A, Miszkiewicz J, Darżynkiewicz ZM, Tyras M, Stankiewicz-Drogoń A, Trylska J, Darżynkiewicz E, Grzela R. Development of bis-ANS-based modified fluorescence titration assay for IFIT/RNA studies Biochem Biophys Res Commun. 2020 Dec 10;533(3):391-396.
2: Mazumdar M, Parrack PK, Mukhopadhyay K, Bhattacharyya B. Bis-ANS as a specific inhibitor for microtubule-associated protein induced assembly of tubulin Biochemistry. 1992 Jul 21;31(28):6470-4.
3: Kamal MZ, Ali J, Rao NM. Binding of bis-ANS to Bacillus subtilis lipase: a combined computational and experimental investigation Biochim Biophys Acta. 2013 Aug;1834(8):1501-9.
4: Hawe A, Poole R, Jiskoot W. Misconceptions over Förster resonance energy transfer between proteins and ANS/bis-ANS: Direct excitation dominates dye fluorescence Anal Biochem. 2010 Jun 1;401(1):99-106.
5: Hawe A, Rispens T, Herron JN, Jiskoot W. Probing bis-ANS binding sites of different affinity on aggregated IgG by steady-state fluorescence, time-resolved fluorescence and isothermal titration calorimetry J Pharm Sci. 2011 Apr;100(4):1294-305.
6: Takashi R, Tonomura Y, Morales MF. 4,4′-Bis (1-anilinonaphthalene 8-sulfonate) (bis-ANS): a new probe of the active site of myosin Proc Natl Acad Sci U S A. 1977 Jun;74(6):2334-8.
7: Bothra A, Bhattacharyya A, Mukhopadhyay C, Bhattacharyya K, Roy S. A fluorescence spectroscopic and molecular dynamics study of bis-ANS/protein interaction J Biomol Struct Dyn. 1998 Apr;15(5):959-66.
8: Mozes E, Hunya A, Toth A, Ayaydin F, Penke B, Datki ZL. A novel application of the fluorescent dye bis-ANS for labeling neurons in acute brain slices Brain Res Bull. 2011 Oct 10;86(3-4):217-21.
9: Smoot AL, Panda M, Brazil BT, Buckle AM, Fersht AR, Horowitz PM. The binding of bis-ANS to the isolated GroEL apical domain fragment induces the formation of a folding intermediate with increased hydrophobic surface not observed in tetradecameric GroEL Biochemistry. 2001 Apr 10;40(14):4484-92.
10: Höger-Weinmüller M. „Bis ans Ende der Welt und über den Rand – mit Adolf Wölfli“ : Ein Rückblick auf die Ausstellung in der Villa Stuck, München, zu sehen von 29.4. bis 15.08.2021 Neuropsychiatr. 2021 Dec;35(4):210-212.

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