Alkaline Phosphatase Assay Buffer (10X)


Catalog Number: B2010944 (50 mL)
Alkaline Phosphatase Assay Buffer (10X) is a highly pure an alkaline phosphatase buffer for AP detection systems on membranes. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other biochemical and immunological applications. Custom bulk amounts of this product are available upon request.

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Alkaline Phosphatase Assay Buffer (10X)
Catalog number: B2010944
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mL
Molecular Weight or Concentration: na
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: AP Assay Buffer
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Sun J, Zhao J, Bao X, Wang Q, Yang X. Alkaline Phosphatase Assay Based on the Chromogenic Interaction of Diethanolamine with 4-Aminophenol Anal Chem. 2018 May 15;90(10):6339-6345.
2: Olczak M, Szulc B. Modified secreted alkaline phosphatase as an improved reporter protein for N-glycosylation analysis PLoS One. 2021 May 25;16(5):e0251805.
3: Gong H, Little G, Cradduck M, Draney DR, Padhye N, Olive DM. Alkaline phosphatase assay using a near-infrared fluorescent substrate merocyanine 700 phosphate Talanta. 2011 May 15;84(3):941-6.
4: Takayanagi T, Mine M, Mizuguchi H. Capillary Electrophoresis/Dynamic Frontal Analysis for the Enzyme Assay of 4-Nitrophenyl Phosphate with Alkaline Phosphatase Anal Sci. 2020 Jul 10;36(7):829-834.
5: Obayashi Y, Iino R, Noji H. A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate Analyst. 2015 Aug 7;140(15):5065-73.
6: Harbron S, Eggelte HJ, Fisher M, Rabin BR. Amplified assay of alkaline phosphatase using flavin-adenine dinucleotide phosphate as substrate Anal Biochem. 1992 Oct;206(1):119-24.
7: Schumann G, Dominick HC, Hellmann D, Klauke R, Möckesch M, Stekel H, von Schenck H, Kraft M, Nagel R, Hänseler E. Alkaline phosphatase activity: new assay for the Reflotron system. Results of the evaluation in eight clinical laboratories Clin Chem Lab Med. 2001 Jan;39(1):71-8.
8: Lewandrowski K, Lee-Lewandrowski E, Bowers GN Jr, McComb RB. Investigation of N-methyl-D-glucamine buffer for assay of alkaline phosphatase in serum Clin Chem. 1992 Nov;38(11):2286-94.
9: Khatun S, Biswas S, Mahanta AK, Joseph MM, Vidyalekshmi MS, Podder A, Maiti P, Maiti KK, Bhuniya S. Biocompatible fluorescent probe for detecting mitochondrial alkaline phosphatase activity in live cells J Photochem Photobiol B. 2020 Nov;212:112043.
10: Ueno H, Kato M, Minagawa Y, Hirose Y, Noji H. Elucidation and control of low and high active populations of alkaline phosphatase molecules for quantitative digital bioassay Protein Sci. 2021 Aug;30(8):1628-1639.

Products Related to Alkaline Phosphatase Assay Buffer (10X): Buffers & Solutions

Additional information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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