ADAMTS-4/Aggrecanase FRET Substrate

Product Description

ADAMTS-4/Aggrecanase FRET Substrate
Catalog number: B2011377
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 mg
Molecular Weight or Concentration: 1644.8 g/mol
Supplied as: Lyophilized
Applications: molecular tool for various biochemical applications
Storage: -20 °C
Keywords: ADAMTS-4/Aggrecanase FRET Substrate, WAAG-3R
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Lauer-Fields JL, Minond D, Sritharan T, Kashiwagi M, Nagase H, Fields GB. Substrate conformation modulates aggrecanase (ADAMTS-4) affinity and sequence specificity. Suggestion of a common topological specificity for functionally diverse proteases J Biol Chem. 2007 Jan 5;282(1):142-50.
2: Fowkes MM, Lim NH. Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted Mutants Methods Mol Biol. 2020;2043:75-91.
3: Roberts S, Evans H, Wright K, van Niekerk L, Caterson B, Richardson JB, Kumar KH, Kuiper JH. ADAMTS-4 activity in synovial fluid as a biomarker of inflammation and effusion Osteoarthritis Cartilage. 2015 Sep;23(9):1622-6.
4: Tortorella M, Pratta M, Liu RQ, Abbaszade I, Ross H, Burn T, Arner E. The thrombospondin motif of aggrecanase-1 (ADAMTS-4) is critical for aggrecan substrate recognition and cleavage J Biol Chem. 2000 Aug 18;275(33):25791-7.
5: Wittwer AJ, Hills RL, Keith RH, Munie GE, Arner EC, Anglin CP, Malfait AM, Tortorella MD. Substrate-dependent inhibition kinetics of an active site-directed inhibitor of ADAMTS-4 (Aggrecanase 1) Biochemistry. 2007 May 29;46(21):6393-401.
6: Santamaria S, Yamamoto K, Teraz-Orosz A, Koch C, Apte SS, de Groot R, Lane DA, Ahnström J. Exosites in Hypervariable Loops of ADAMTS Spacer Domains control Substrate Recognition and Proteolysis Sci Rep. 2019 Jul 29;9(1):10914.
7: Santamaria S, Cuffaro D, Nuti E, Ciccone L, Tuccinardi T, Liva F, D’Andrea F, de Groot R, Rossello A, Ahnström J. Exosite inhibition of ADAMTS-5 by a glycoconjugated arylsulfonamide Sci Rep. 2021 Jan 13;11(1):949.
8: Wainwright SD, Bondeson J, Caterson B, Hughes CE. ADAMTS-4_v1 is a splice variant of ADAMTS-4 that is expressed as a protein in human synovium and cleaves aggrecan at the interglobular domain Arthritis Rheum. 2013 Nov;65(11):2866-75.
9: Tortorella MD, Arner EC, Hills R, Easton A, Korte-Sarfaty J, Fok K, Wittwer AJ, Liu RQ, Malfait AM. Alpha2-macroglobulin is a novel substrate for ADAMTS-4 and ADAMTS-5 and represents an endogenous inhibitor of these enzymes J Biol Chem. 2004 Apr 23;279(17):17554-61.
10: Moncada-Pazos A, Obaya AJ, Viloria CG, López-Otín C, Cal S. The nutraceutical flavonoid luteolin inhibits ADAMTS-4 and ADAMTS-5 aggrecanase activities J Mol Med (Berl). 2011 Jun;89(6):611-9.

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