6-Carboxyfluorescein Diacetate

Product Description

6-Carboxyfluorescein Diacetate
Catalog number: B2011020
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 mg
Molecular Weight or Concentration: 460.4 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical studies
Storage: -20C
Keywords: 6-CFDA, 3′,6′-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-6-carboxylic Acid
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Quah BJ, Warren HS, Parish CR. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester Nat Protoc. 2007;2(9):2049-56.
2: Quah BJ, Parish CR. The use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte proliferation J Vis Exp. 2010 Oct 12;(44):2259.
3: Terrén I, Orrantia A, Vitallé J, Zenarruzabeitia O, Borrego F. CFSE dilution to study human T and NK cell proliferation in vitro Methods Enzymol. 2020;631:239-255.
4: Lyons AB, Blake SJ, Doherty KV. Flow cytometric analysis of cell division by dilution of CFSE and related dyes Curr Protoc Cytom. 2013;Chapter 9:Unit9.11.
5: Hawkins ED, Hommel M, Turner ML, Battye FL, Markham JF, Hodgkin PD. Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data Nat Protoc. 2007;2(9):2057-67.
6: Gnoula C, Guissou I, Dubois J, Duez P. 5(6)-Carboxyfluorescein diacetate as an indicator of Caenorhabditis elegans viability for the development of an in vitro anthelmintic drug assay Talanta. 2007 Mar 30;71(5):1886-92.
7: Quah BJ, Parish CR. New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes J Immunol Methods. 2012 May 31;379(1-2):1-14.
8: Brunet A, Aslam T, Bradley M. Separating the isomers–efficient synthesis of the N-hydroxysuccinimide esters of 5 and 6-carboxyfluorescein diacetate and 5 and 6-carboxyrhodamine B Bioorg Med Chem Lett. 2014 Jul 15;24(14):3186-8.
9: Qiu C, Zhang D, Chi Y, Chen Q, Xu L, Xie Q. Clinical significance of 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester-labeled microspheres for detecting endothelial progenitor cells in human peripheral blood Exp Ther Med. 2017 Aug;14(2):1659-1664.
10: Azarsiz E, Karaca N, Ergun B, Durmuscan M, Kutukculer N, Aksu G. In vitro T lymphocyte proliferation by carboxyfluorescein diacetate succinimidyl ester method is helpful in diagnosing and managing primary immunodeficiencies J Clin Lab Anal. 2018 Jan;32(1):e22216.

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