5-Iodoacetamidofluorescein (5-IAF)

Product Description

5-Iodoacetamidofluorescein (5-IAF)
Catalog number: B2011353
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 515.3 g/mol
Supplied as: Lyophilized
Applications: molecular tool for various biochemical applications
Storage: -20 °C
Keywords: 5-IAF [5-Iodoacetamidofluorescein]
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Sotgia S, Pisanu E, Pintus G, Erre GL, Pinna GA, Deiana L, Carru C, Zinellu A. Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection PLoS One. 2013 Jul 29;8(7):e70374.
2: Steinberg M, Karlish SJ. Studies on conformational changes in Na,K-ATPase labeled with 5-iodoacetamidofluorescein J Biol Chem. 1989 Feb 15;264(5):2726-34.
3: Hellen EH, Pratap PR. Nucleotide binding to IAF-labelled Na+/K(+)-ATPase measured by steady state fluorescence quenching by TNP-ADP Biophys Chem. 1997 Dec 1;69(2-3):107-24.
4: Schmidt M, Tschödrich-Rotter M, Peters R, Krohne G. Properties of fluorescently labeled Xenopus lamin A in vivo Eur J Cell Biol. 1994 Oct;65(1):70-81.
5: Alhathal N, San Gabriel M, Zini A. Beneficial effects of microsurgical varicocoelectomy on sperm maturation, DNA fragmentation, and nuclear sulfhydryl groups: a prospective trial Andrology. 2016 Nov;4(6):1204-1208.
6: Liu P, Huang J, Zhong L. Role and mechanism of homocysteine in affecting hepatic protein-tyrosine phosphatase 1B Biochim Biophys Acta Gen Subj. 2019 May;1863(5):941-949.
7: Muddukrishna SN, Chen A, Qi P, Smolenski MA. Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique Appl Radiat Isot. 1995 Oct;46(10):1015-26.
8: Kapakos JG, Steinberg M. 5-Iodoacetamidofluorescein-labeled (Na,K)-ATPase. Steady-state fluorescence during turnover J Biol Chem. 1986 Feb 15;261(5):2090-5.
9: Musenga A, Mandrioli R, Bonifazi P, Kenndler E, Pompei A, Raggi MA. Sensitive and selective determination of glutathione in probiotic bacteria by capillary electrophoresis-laser induced fluorescence Anal Bioanal Chem. 2007 Feb;387(3):917-24.
10: Bock PE, Shore JD, Tans G, Griffin JH. Protein-protein interactions in contact activation of blood coagulation. Binding of high molecular weight kininogen and the 5-(iodoacetamido) fluorescein-labeled kininogen light chain to prekallikrein, kallikrein, and the separated kallikrein heavy and light chains J Biol Chem. 1985 Oct 15;260(23):12434-43.

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