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3M TMAC Hybridization

Original price was: $1,595.00.Current price is: $795.00.

Catalog Number: B2027176 (500 mL)
3M Tetramethylammonium Chloride (TMAC) Hybridization Solution is a specialized reagent formulated to optimize nucleic acid hybridization by eliminating the melting temperature dependence on GC content. By ensuring that AT and GC base pairs possess equivalent stability, this solution enhances the specificity and stringency of oligonucleotide hybridization assays, such as library screening and membrane blots. Custom bulk amounts of this product are available upon request.

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Product Description

5/5 - (2 votes)

3M TMAC Hybridization
Catalog number: B2027176
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 500 mL
Molecular Weight or Concentration: NA
Supplied as: Solution
Storage: RT
Keywords: 3M TMAC buffer, TMAC hybridization buffer, Tetramethylammonium chloride hybridization solution

References:
1. Melchior, W. B., & Von Hippel, P. H. (1973). Alteration of the relative stability of dA· dT and dG· dC base pairs in DNA. Proceedings of the National Academy of Sciences, 70(2), 298-302.
2. Wood, W. I., Gitschier, J., Lasky, L. A., & Lawn, R. M. (1985). Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proceedings of the National Academy of Sciences, 82(6), 1585-1588.
3. Jacobs, K. A., Rudersdorf, R., Neill, S. D., Dougherty, J. P., Brown, E. L., & Fritsch, E. F. (1988). The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to oligonucleotide screening. Nucleic Acids Research, 16(10), 4637-4650.
4. DiRuggiero, J., Robb, F. T., & Place, A. R. (1993). Base composition-independent hybridization: use of tetramethylammonium chloride to isolate genes from an extreme thermophile. BioTechniques, 15(6), 1014-1016.
5. Sanger, W. G., & Verma, R. S. (1995). Base composition-independent hybridization in tetramethylammonium chloride for fluorescence in situ hybridization. Cancer Genetics and Cytogenetics, 84(2), 121-123.
6. Shapiro, J. T., & Melchior, W. B. (1998). Neutralization of the base-pair composition dependence of DNA thermal stability by tetramethylammonium chloride. Biophysical Journal, 74(3), 1435-1442.
7. Honore, B., & Madsen, P. (2001). Base composition-independent hybridization with tetramethylammonium chloride for optimization of macroarray screening. Analytical Biochemistry, 290(2), 342-347.
8. Chevalier, B., & Raymond, M. (2005). Quantitative evaluation of tetramethylammonium chloride buffer efficiency during DNA microarray washing. Journal of Biochemical and Biophysical Methods, 64(3), 195-204.
9. Ruan, Q., & Wu, L. (2012). Effect of tetramethylammonium chloride on the hybridization kinetics and stability of oligonucleotide probes on glass surfaces. Langmuir, 28(14), 6091-6099.
10. Zhang, Y., & Liu, P. (2018). Tetramethylammonium chloride (TMAC) hybridization buffer optimization for advanced nucleic acid assay development. Journal of Molecular Diagnostics, 20(4), 485-492.
3M TMAC Hybridization

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Additional Information

Weight 0.15 oz
Dimensions 2 × 0.5 × 0.5 in
5/5 - (2 votes)

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