2′,7′-Dichlorofluorescein Fluorescent Indicator

Product Description

2′,7′-Dichlorofluorescein Fluorescent Indicator
Catalog number: B2012743
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 mg
Molecular Weight or Concentration: 401.2 g/mol
Supplied as: Lyophilized Powder
Applications: molecular tool for various biochemical applications
Storage: RT
Keywords: 2′,7′-Dichlorofluorescein [for Fluorescent indicator]
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Reiniers MJ, de Haan LR, Reeskamp LF, Broekgaarden M, Hoekstra R, van Golen RF, Heger M. Optimal Use of 2′,7′-Dichlorofluorescein Diacetate in Cultured Hepatocytes Methods Mol Biol. 2022;2451:721-747.
2: Daghastanli NA, Itri R, Baptista MS. Singlet oxygen reacts with 2′,7′-dichlorodihydrofluorescein and contributes to the formation of 2′,7′-dichlorofluorescein Photochem Photobiol. 2008 Sep-Oct;84(5):1238-43.
3: Bilski P, Belanger AG, Chignell CF. Photosensitized oxidation of 2′,7′-dichlorofluorescin: singlet oxygen does not contribute to the formation of fluorescent oxidation product 2′,7′-dichlorofluorescein Free Radic Biol Med. 2002 Oct 1;33(7):938-46.
4: Chen X, Zhong Z, Xu Z, Chen L, Wang Y. 2′,7′-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy Free Radic Res. 2010 Jun;44(6):587-604.
5: Vaglienti MV, Subirada PV, Barcelona PF, Bonacci G, Sanchez MC. Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells J Vis Exp. 2022 May 13;(183).
6: Yao H, Jockusch RA. Fluorescence and electronic action spectroscopy of mass-selected gas-phase fluorescein, 2′,7′-dichlorofluorescein, and 2′,7′-difluorofluorescein ions J Phys Chem A. 2013 Feb 14;117(6):1351-9.
7: Zhu H, Bannenberg GL, Moldéus P, Shertzer HG. Oxidation pathways for the intracellular probe 2′,7′-dichlorofluorescein Arch Toxicol. 1994;68(9):582-7.
8: Rota C, Chignell CF, Mason RP. Evidence for free radical formation during the oxidation of 2′-7′-dichlorofluorescin to the fluorescent dye 2′-7′-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements Free Radic Biol Med. 1999 Oct;27(7-8):873-81.
9: Kalinich JF, Ramakrishnan N, McClain DE. The antioxidant Trolox enhances the oxidation of 2′,7′-dichlorofluorescin to 2′,7′-dichlorofluorescein Free Radic Res. 1997 Jan;26(1):37-47.
10: Marchesi E, Rota C, Fann YC, Chignell CF, Mason RP. Photoreduction of the fluorescent dye 2′-7′-dichlorofluorescein: a spin trapping and direct electron spin resonance study with implications for oxidative stress measurements Free Radic Biol Med. 1999 Jan;26(1-2):148-61.

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