β-Glucosidase (Agrobacterium sp.)

Description

β-Glucosidase (Agrobacterium sp.)
Catalog number: B2014491
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 U
Molecular Weight or Concentration: 52.2 kDa
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: 2-8°C
Keywords: beta-glucosidase; beta-D-glucoside glucohydrolase
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Mayer C, Zechel DL, Reid SP, Warren RA, Withers SG. The E358S mutant of Agrobacterium sp. beta-glucosidase is a greatly improved glycosynthase FEBS Lett. 2000 Jan 21;466(1):40-4.
2: Shim JH, Chen HM, Rich JR, Goddard-Borger ED, Withers SG. Directed evolution of a β-glycosidase from Agrobacterium sp. to enhance its glycosynthase activity toward C3-modified donor sugars Protein Eng Des Sel. 2012 Sep;25(9):465-72.
3: Gao H, Xie F, Zhang W, Tian J, Zou C, Jia C, Jin M, Huang J, Chang Z, Yang X, Jiang D. Characterization and improvement of curdlan produced by a high-yield mutant of Agrobacterium sp. ATCC 31749 based on whole-genome analysis Carbohydr Polym. 2020 Oct 1;245:116486.
4: Domi B, Rumbo C, García-Tojal J, Elena Sima L, Negroiu G, Tamayo-Ramos JA. Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules Int J Mol Sci. 2019 Dec 27;21(1):205.
5: Lawson SL, Warren RA, Withers SG. Mechanistic consequences of replacing the active-site nucleophile Glu-358 in Agrobacterium sp. beta-glucosidase with a cysteine residue Biochem J. 1998 Feb 15;330 ( Pt 1)(Pt 1):203-9.
6: Green DE, Bowen ML, Scott LE, Storr T, Merkel M, Böhmerle K, Thompson KH, Patrick BO, Schugar HJ, Orvig C. In vitro studies of 3-hydroxy-4-pyridinones and their glycosylated derivatives as potential agents for Alzheimer’s disease Dalton Trans. 2010 Feb 14;39(6):1604-15.
7: Tolborg JF, Petersen L, Jensen KJ, Mayer C, Jakeman DL, Warren RA, Withers SG. Solid-phase oligosaccharide and glycopeptide synthesis using glycosynthases J Org Chem. 2002 Jun 14;67(12):4143-9.
8: Hermetter A, Scholze H, Stütz AE, Withers SG, Wrodnigg TM. Powerful probes for glycosidases: novel, fluorescently tagged glycosidase inhibitors Bioorg Med Chem Lett. 2001 May 21;11(10):1339-42.
9: Wrodnigg TM, Withers SG, Stütz AE. Novel, lipophilic derivatives of 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) are powerful beta-glucosidase inhibitors Bioorg Med Chem Lett. 2001 Apr 23;11(8):1063-4.
10: Wrodnigg TM, Diness F, Gruber C, Häusler H, Lundt I, Rupitz K, Steiner AJ, Stütz AE, Tarling CA, Withers SG, Wölfler H. Probing the aglycon binding site of a beta-glucosidase: a collection of C-1-modified 2,5-dideoxy-2,5-imino-D-mannitol derivatives and their structure-activity relationships as competitive inhibitors Bioorg Med Chem. 2004 Jul 1;12(13):3485-95.

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