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A new interesting article has been published in J Clin Pathol. 2019 Dec; 72(12):830-835. doi: 10.1136/jclinpath-2019-205935. Epub 2019 Jun 23. Comparative Study and titled:

Microsatellite instability diagnosis using the fully automated Idylla platform: feasibility study of an in-house rapid molecular testing ancillary …

Authors of this article are:

Samaison L, Grall M, Staroz F, Uguen A.

A summary of the article is shown below:

AIM: To study the performances of the Idylla MSI Assay in the diagnosis of microsatellite instability (MSI) or microsatellite stability (MSS).METHODS: We selected 12 tumour samples previously tested for MSI focusing on cases with discrepant results between MLH1, PMS2, MSH2 and MSH6 immunohistochemistry and microsatellite molecular analyses (five cases) or doubtful immunohistochemistry (two cases). Idylla MSI Assay was compared with retrospective immunohistochemistry and molecular results.RESULTS: Idylla MSI Assay showed an almost perfect concordance with microsatellite analysis results previously obtained (only one case with not fully conclusive analysis due to sample exhaustion). The full molecular analysis took less than 150 min per sample and revealed no mutation in any of the seven microsatellite sequences in five MSS samples and four to six mutated ones in seven MSI-High samples.CONCLUSION: At the era when the determination of MSI/MSS status is becoming important for rapid treatment choices, the Idylla MSI Assay consists of a valuable easy-to-perform diagnostic tool that allows, complementary to MLH1, PMS2, MSH2 and MSH6 immunohistochemistry, the diagnosis of MSI/MSS status in a single day.© Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ.

Check out the article’s website on Pubmed for more information:

[link-preview url=https://www.ncbi.nlm.nih.gov/pubmed/31235541 forceshot=true]

This article is a good source of information and a good way to become familiar with topics such as: Adult; Aged; Automation, Laboratory; Biomarkers, Tumor; Clinical Decision-Making; Feasibility Studies; Female; Humans; Immunohistochemistry; Male; Middle Aged; Molecular Diagnostic Techniques; Neoplasms; Predictive Value of Tests; Prognosis; Retrospective Studies; Workflow.

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