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A new interesting article has been published in Int J Lab Hematol. 2019 Dec 10. doi: 10.1111/ijlh.13134. and titled:

Magnesium-dependent activated partial thromboplastin time assay-Simple method for lupus anticoagulant detection.

Authors of this article are:

Tokutake T, Ieko M, Naito S, Yoshida M, Baba H, Kobayashi H, Ishida F.

A summary of the article is shown below:

INTRODUCTION: Detection of lupus anticoagulant (LA), an antiphospholipid (aPL) antibody, in a clotting time test is an important finding for diagnosis of antiphospholipid syndrome (APS). However, confirmation of LA requires several different testing procedures, some of which can be difficult and require time. We report here a simple and highly specific method for detecting LA.MATERIALS AND METHODS: We examined 66 plasma samples obtained from LA-positive (LA) and 75 from LA-negative (non-LA) subjects, which included patients with acquired hemophilia and coagulation disorders, as well as from 43 healthy volunteer samples as normal controls. Activated partial thromboplastin time (APTT) was determined by adding 20 mmol of CaCl2 (Ca-APTT) or 25 mmol of a mixture of Mg and Ca (Mg-APTT). The ratio of Mg-APTT/Ca-APTT was then calculated and used as the Mg/Ca Index.RESULTS: The Mg/Ca Index value for the LA group was significantly lower than that for the non-LA and normal control groups (P < .0001). When the cutoff value of the Mg/Ca Index was less than 1.00, the sensitivity of LA determination using the Mg-APTT assay was 80.3%, while specificity was 100%.CONCLUSION: Our findings indicate that the present Mg-APTT assay is a simple yet highly specific method for LA detection.© 2019 John Wiley & Sons Ltd.
Check out the article’s website on Pubmed for more information:

[link-preview url=https://www.ncbi.nlm.nih.gov/pubmed/31821738 forceshot=true]

This article is a good source of information and a good way to become familiar with topics such as: activated partial thromboplastin time (APTT); antiphospholipid syndrome (APS); lupus anticoagulant (LA); magnesium.

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