Thrombin Substrate III Fluorogenic

Description

Thrombin Substrate III Fluorogenic
Catalog number: B2014117
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 681.78 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: Thrombin Substrate III, Fluorogenic, Benzoyl-FVR-AMC
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Triplett DA, Banez EI, Harms CS. Fluorogenic substrate assay of antithrombin III: a clinical study Thromb Res. 1983 Sep 15;31(6):855-62.
2: Choo IH, Didisheim P, Doerge ML, Johnson ML, Bach ML, Melchert LM, Johnson WJ, Taylor WF. Evaluation of a heparin assay method using a fluorogenic synthetic peptide substrate for thrombin Thromb Res. 1982 Jan 1-15;25(1-2):115-23.
3: Fareed J, Messmore HL, Walenga JM, Bermes EW Jr. Synthetic peptide substrates in hemostatic testing Crit Rev Clin Lab Sci. 1983;19(2):71-134.
4: Yaron JR, Ambadapadi S, Zhang L, Lucas A. Kinetic Measurement of Serpin Inhibitory Activity by Real-Time Fluorogenic Biochemical Assay Methods Mol Biol. 2018;1826:65-71.
5: Kawabata S, Morita T, Iwanaga S, Igarashi H. Difference in enzymatic properties between alpha-thrombin-staphylocoagulase complex and free alpha-thrombin J Biochem. 1985 Apr;97(4):1073-8.
6: Naski MC, Shafer JA. Alpha-thrombin-catalyzed hydrolysis of fibrin I. Alternative binding modes and the accessibility of the active site in fibrin I-bound alpha-thrombin J Biol Chem. 1990 Jan 25;265(3):1401-7.
7: Naski MC, Fenton JW 2nd, Maraganore JM, Olson ST, Shafer JA. The COOH-terminal domain of hirudin. An exosite-directed competitive inhibitor of the action of alpha-thrombin on fibrinogen J Biol Chem. 1990 Aug 15;265(23):13484-9.
8: Sano M, Tamada Y, Niwa K, Morita T, Yoshino G. Sulfated sericin is a novel anticoagulant influencing the blood coagulation cascade J Biomater Sci Polym Ed. 2009;20(5-6):773-83.
9: Kapke GF, Feld RD, Witte DL, Johnson GF. Single-stage automated assay for heparin Clin Chem. 1982 Jul;28(7):1521-4.
10: Patel D, Frelinger J, Goudsmit J, Kim B. In vitro assay for site-specific proteases using bead-attached GFP substrate Biotechniques. 2001 Nov;31(5):1194, 1196, 1198 passim.

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