T4 Polynucleotide Kinase (3′-phosphatase free)

Description

T4 Polynucleotide Kinase (3′-phosphatase free)
Catalog number: B2013431
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 200 units
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: kinase, t4 polynucleotide, 3′-phosphatase-free
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Tao J, Liu Z, Zhu Z, Zhang Y, Wang H, Pang P, Yang C, Yang W. Electrochemical detection of T4 polynucleotide kinase activity based on magnetic Fe(3)O(4)@TiO(2) nanoparticles triggered by a rolling circle amplification strategy Talanta. 2022 May 1;241:123272.
2: Wei L, Kong X, Wang M, Zhang Y, Pan R, Cheng Y, Lv Z, Zhou J, Ming J. A label-free T4 polynucleotide kinase fluorescence sensor based on split dimeric G-quadruplex and ligation-induced dimeric G-quadruplex/thioflavin T conformation Anal Bioanal Chem. 2022 Nov;414(27):7923-7933.
3: Folk WR. Phage T4 polynucleotide kinase Gene Amplif Anal. 1981;2:299-311.
4: Yang J, He G, Wu W, Deng W, Tan Y, Xie Q. Sensitive photoelectrochemical determination of T4 polynucleotide kinase using AuNPs/SnS(2)/ZnIn(2)S(4) photoactive material and enzymatic reaction-induced DNA structure switch strategy Talanta. 2022 Nov 1;249:123660.
5: Zhang T, Shen Y, Ge J, Wang W, Qu L, Li Z. A highly sensitive fluorescence method for the detection of T4 polynucleotide kinase phosphatase based on polydopamine nanotubes Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 15;267(Pt 2):120594.
6: Gao M, Guo J, Song Y, Zhu Z, Yang CJ. Detection of T4 Polynucleotide Kinase via Allosteric Aptamer Probe Platform ACS Appl Mater Interfaces. 2017 Nov 8;9(44):38356-38363.
7: Green MR, Sambrook J. Labeling the 5′ Termini of DNA with Bacteriophage T4 Polynucleotide Kinase Cold Spring Harb Protoc. 2021 Aug 2;2021(8).
8: Cameron V, Soltis D, Uhlenbeck OC. Polynucleotide kinase from a T4 mutant which lacks the 3′ phosphatase activity Nucleic Acids Res. 1978 Mar;5(3):825-33.
9: Midgley CA, Murray NE. T4 polynucleotide kinase; cloning of the gene (pseT) and amplification of its product EMBO J. 1985 Oct;4(10):2695-703.
10: Zhang L, Fan W, Jia D, Feng Q, Ren W, Liu C. Microchamber-Free Digital Flow Cytometric Analysis of T4 Polynucleotide Kinase Phosphatase Based on Single-Enzyme-to-Single-Bead Space-Confined Reaction Anal Chem. 2021 Nov 9;93(44):14828-14836.

Products Related to T4 Polynucleotide Kinase (3′-phosphatase free) can be found at Enzymes